Polyester Hydrolase Screening Service

inquiry

Polyester hydrolase screening is used to compare enzyme candidates for activity on PET, PET-like model substrates, or related polyester materials under controlled laboratory conditions. The service is suitable when a project has multiple candidate enzymes, expression samples, metagenomic hits, or purified proteins that need to be triaged before more detailed validation.

The screening design can be adjusted according to candidate format, substrate relevance, and the level of analytical confirmation needed. Depending on the project stage, screening may be combined with PET hydrolysis activity assays, candidate validation, product analysis, or condition profiling.

Service Scope

The screening workflow is designed to generate comparable activity evidence across a panel of candidates. It does not assume that every candidate will be active on PET, and it does not treat activity on a soluble model substrate as equivalent to hydrolysis of solid PET. The substrate strategy, readout, and hit criteria are defined according to the research question.

Projects may start from client-supplied enzymes, crude expression samples, sequence-mined candidates, literature-derived enzymes, or preselected polyesterase panels. The output is typically a ranked set of candidates with supporting assay data and recommendations for follow-up testing.

For background on PET hydrolase terminology, assay design, and research boundaries, see the PET Hydrolase Research Guide.

Related Testing Options

PET Hydrolysis Activity Assay Used when one or a small number of enzyme samples need direct activity testing on a defined PET substrate.

PET Hydrolase Candidate Validation Used when a sequence, clone, or screening hit needs expression, purification, QC, and activity confirmation.

PET Substrate and Hydrolysis Product Analysis Used when product identity, product level, or substrate-related interpretation is central to the project.

Polyester Hydrolase Condition Profiling Used after an active candidate is identified and needs evaluation across pH, temperature, time, or substrate conditions.

Candidate Types That Can Be Screened

Candidate Format	Typical Use	Information Needed
Purified enzyme panel	Direct comparison of candidates under the same substrate and reaction conditions.	Protein concentration, buffer, storage condition, purity if known, and number of candidates.
Crude expression samples	Early triage when purification has not yet been completed.	Host system, sample matrix, expression scale, expected target protein, and background components.
Metagenomic or sequence-mined hits	Evaluation of candidates identified from sequence analysis or library screening.	Sequence files, annotation summary, predicted family, and expression status.
Client-selected enzyme variants	Comparison of engineered or literature-derived candidates.	Variant identifiers, sequence differences, expression format, and intended comparison criteria.

Substrate Strategy

Substrate selection determines what the screen can and cannot conclude. A model substrate may be useful for broad esterase or polyesterase triage, while PET film, PET powder, or amorphous PET is more relevant when the project requires evidence of PET hydrolysis. The substrate should be selected before hit criteria are defined.

PET-like model substrates: useful for fast triage, but positive results require follow-up on PET or a more relevant polyester substrate.
PET film or powder: useful for research questions focused on PET hydrolysis under defined conditions.
Related polyester substrates: useful when the goal is broader polyester hydrolase activity rather than PET alone.
Client-supplied substrates: possible when material history and handling requirements are provided.

Screening Workflow

1Project intake
Define candidate format, substrate type, assay goal, and expected deliverables.
2Primary screen
Run a controlled assay to identify candidates with measurable activity under selected conditions.
3Secondary confirmation
Retest promising hits with a more specific substrate, replicate design, or product readout.
4Hit interpretation
Rank candidates based on signal strength, reproducibility, substrate relevance, and assay limitations.
5Follow-up plan
Recommend validation, condition profiling, or product analysis for selected candidates.

Hit Criteria and Secondary Confirmation

Hit criteria should be defined before screening begins. A candidate may be called a preliminary hit when its signal is above the selected background threshold, reproducible across replicates, and consistent with the expected product or endpoint. For PET-focused projects, secondary confirmation usually requires a more specific readout than a general esterase assay.

Secondary confirmation may include repeat testing, product quantification, substrate comparison, or activity confirmation on PET rather than a model substrate. This step is important when results will guide cloning, purification, engineering, or further investment in a candidate.

Deliverables

Screening design summary, including substrate, reaction conditions, controls, and readout.
Candidate activity table with raw or processed readout values where applicable.
Preliminary hit list with ranking rationale and notes on assay limitations.
Secondary confirmation results for selected candidates, if included in the project scope.
Recommendations for candidate validation, product analysis, or condition profiling.

Reference Reagent

When a comparison or method-development reagent is useful, Leaf-branch Compost Poly(ethylene terephthalate) Hydrolase may be considered as a PET hydrolase research reagent. It is not presented here as part of a broad PETase product family.

Project note: Screening results depend on the substrate, enzyme format, reaction conditions, and analytical endpoint. The data are intended to support research and development decisions, including candidate triage and selection of follow-up assays.

Request Polyester Hydrolase Screening

FAQs About Polyester Hydrolase Screening

Q: When should I choose screening instead of a single activity assay?

A: Screening is more suitable when multiple enzyme candidates need to be compared under the same substrate, reaction conditions, and readout. A single activity assay is more suitable when only one or a small number of samples need direct PET hydrolysis confirmation.
Q: Can PETase variants be supplied through this service?

A: No. This service is not positioned as a PETase variant supply page. It supports screening of client-supplied or project-defined candidates under research conditions.
Q: Can crude enzyme samples be screened?

A: In some cases, yes. Crude samples require careful controls because sample matrix components can affect the assay signal or product analysis.
Q: Does activity on a model substrate count as PET hydrolysis?

A: Not by itself. Model-substrate activity can support early triage, but PET hydrolysis should be confirmed with a defined PET substrate and an appropriate readout.
Q: What happens after a screening hit is found?

A: Follow-up may include candidate validation, repeat activity testing, product analysis, condition profiling, or expression and purification work depending on the project goal.

First Name:

Last Name:

Email *

Phone Number:

Company/Institution:

Country or Region:

Quantity:

Services & Products of Interested *

Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

Services

Online Inquiry