Enzyme Activity Measurement for Oxidoreductases Acting on Carbon Using Spectrophotometric Assays

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Creative Enzymes proudly delivers enzyme activity measurement specialized for oxidoreductases that act on carbon groups. Our unique service determines accurate and reproducible enzyme activities using the cutting edge spectrophotometric equipment. The oxidoreductases that act on carbon groups, designated as EC 1.1, EC 1.2, and EC 1.3, contain a large number of enzyme members and play essential roles in many industries. Therefore, quantification of the enzyme activity is of high complexity. Creative Enzymes is gladly braced by enzymology experts who have experiences on activity tests for all kinds of oxidoreductases. With the combination of the talented team and top-quality instruments, our activity assay services has a remarkable advantage.

Oxidoreductases are often named as “oxidase”, “reductase”, or “dehydrogenase”. Nonetheless, they commonly catalyze both oxidation and reduction reactions of the same set of molecules, which are considered as the substrates and products, or the products and substrates, depending on the direction of the reaction. Because most enzymatic oxidation-reduction (redox) reactions are reversible, the equilibrium of the reaction is mainly determined by the redox potential of the substrates and products, the oxidation state of the cofactors, and the concentrations of each species. Subsequently, choosing the proper assay method, including the correct reaction agents and cofactors is critical to a successful and reliable enzyme activity measurement. You can count on Creative Enzymes in performing such a demanding task.

Enzyme Activity Measurement for Oxidoreductases Acting on Carbon Using Spectrophotometric Assays Figure: A bacterialalcohol dehydrogenase that metabolizes ethanol. The cofactor NAD is showed in blue.
Reference: Brown, S. D. et. al.,Proc. Natl. Acad. Sci. U. S. A.2011, 108 (33), 13752.

Due to their instrumental functions in metabolism, chemical transformations, and electron transportation, the oxidoreductases acting on carbon groups are prevalent on pharmaceutical, chemical, personal care, animal and pet, environmental, and nutritional applications. Some oxidoreductase enzymes act on the CH-OH groups, designated as EC 1.1, including alcohol dehydrogenase, xylulose reductase, lactate dehydrogenase, choline dehydrogenase, and gluconate dehydrogenase. While some act on aldehyde and the oxo groups, designated as EC 1.2, which include formate dehydrogenase, aldehyde dehydrogenase, retinal dehydrogenase, pyruvate dehydrogenase, vanillin dehydrogenase, oxalate dehydrogenase, carbon monoxide dehydrogenase, and carboxylate dehydrogenase. The others catalyze redox reactions of the CH-CH groups, designated as EC 1.3. For examples, fumarate dehydrogenase, cortisone dehydrogenase, nitroacrylate dehydrogenase, enoyl-coa dehydrogenase, acyl-coa oxidase, arylyl-coa dehydrogenase, galactonolactone dehydrogenase, scuccinate dehydrogenase, and quinolone dehydrogenase. While the category EC 1.17 contains enzymes that act on methylene and methine groups.

We understand that your business could be concentrated on a specific area of the above-mentioned industries or an entirely different need. In any case, your unique request will be satisfied in full with our exceptional power in enzyme activity assays. Creative Enzymes is happy to serve you with activity measurement for any of the oxidoreductases:

EC 1.1 Acting on the CH-OH group of donors

EC 1.2 Acting on the aldehyde or oxo group of donors

EC 1.3 Acting on the CH-CH group of donors

EC 1.17 Acting on CH or CH2 groups

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