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Enzymes Expression in Other Yeasts

It has become evident that the compatibility of the host's biochemical environment with the ability to process and translate the RNA transcript, along with its ability to modify and sustain the translated protein are equally important in choosing the most appropriate host cell for protein expression. In addition to the most common expression system such as Saccharomyces cerevisiae and Pichia pastoris, several other yeast expression systems are under development. The most popular yeast stains include Arxula adeninivorans, Kluyveromyces lactis, Yarrowia lipolytica, and Schizosaccharomyces pombe showing unique advantages that the typical expression systems do not offer. Therefore, to facilitate the pioneering and early-stage research, Creative Enzymes provides enzyme expression services in these yeast systems.

A. adeninivorans seems an attractive host for heterologous gene expression due to competitive characteristics such as thermos-tolerance and halo-tolerance. Besides, the differential morphology-dependent glycosylation and secretion properties further arouse its attractiveness. In the construction process, the recombinant strain is established through the transformation of foreign DNA fragments into the genome, which guarantees the genetic stability. There are already some successful expression examples through A. adeninivorans. For instances, GFP, human has, and IL6, which all demonstrated the promising future of using A. adeninivorans as a heterologous enzyme expression host.

The yeast Kluyveromyces lactis has been studied for decades and has a well-established track record of safe use in various food industry applications. As an expression host, Kluyveromyces lactis is best known for its use in commercial production of the milk-clotting enzyme bovine chymosin. K. lactis has several advantages over other yeast expression systems: easy genetic manipulation, the ability to use both integrative and episomal expression vectors, and the availability of a fully sequenced genome information. Compared to the methylotrophic yeasts, K. lactis is able to grow in standard yeast medium so the fermentation process is safer. In addition, enzymes from K. lactis have GRAS (generally regarded as safe) FDA status, which permits wide use in various food, nutrition, and feed applications.

Due to the approvement for several GRAS industrial processes by FDA, Yarrowia lipolytica has also been demonstrated to be a good alternative expression system. It presents numerous advantages as a heterologous protein expression system. Firstly, it has the inherent ability to secrete high molecular weight enzymes. Secondly, the co-translational translocation pathway contributes forming enzymes with the correct structures and functions. Thirdly, the genome is completely sequenced and annotated. Combined with the well-studied molecular biology, the whole construction process is easy to accomplish.

Schizosaccharomyces pombe is considered as an attractive host for the expression of proteins with complicated structures, such as glycosylated proteins derived from higher animals. Several proteins are reported successfully expressed in Sc. pombe. Sc. pombe has also been used as a host for production of enzymes to mediate biotransformations. Since Sc. pombe expression system is not fully developed yet, many possibilities attract a number of ongoing research on the yeast as a promising expression system.

Creative Enzymes developed these unconventional yeast expression systems to meet a variety of special demands on enzyme production. Our specialized experts provide professional technical support and genuine analysis reports. The molecular biological technologies and extensive experiences guarantee the rapid construction and the successful fermentation process. All you need for the next successful milestone is to trust Creative Enzymes as the development partner and enjoy the excellent services.

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