Biocatalysis Services

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Biocatalysis has emerged as a transformative technology for sustainable chemical synthesis, pharmaceutical manufacturing, and industrial biotechnology. Creative Enzymes offers comprehensive Biocatalysis Services that cover the entire lifecycle of biocatalyst development, from substrate selection and enzyme discovery to reaction route design and large-scale process development. Leveraging extensive expertise in molecular biology, protein engineering, metabolic engineering, and industrial process development, we support clients in designing efficient, robust, and scalable biocatalytic systems tailored to specific application needs. Our integrated platform enables the development of enzymatic and microbial catalysts that deliver high selectivity, improved yields, reduced environmental impact, and cost-effective production. Through close collaboration with academic partners and industry stakeholders, Creative Enzymes provides innovative, reliable, and customized biocatalysis solutions that bridge laboratory research and industrial manufacturing, supporting both early-stage feasibility studies and commercial-scale implementation.

Background: The Role of Biocatalysis in Green Chemistry and Industrial Biotechnology

Biological catalysts include enzymes and whole-cell systems

Biocatalysis refers to the use of natural or engineered biological catalysts—such as enzymes or whole-cell systems—to accelerate chemical reactions with high specificity and efficiency. As industries increasingly seek sustainable alternatives to traditional chemical catalysis, biocatalysis has gained significant attention due to its inherent advantages, including mild reaction conditions, reduced energy consumption, minimal by-product formation, and improved environmental compatibility.

Natural biocatalysts, including enzymes derived from microorganisms, plants, or animals, exhibit remarkable catalytic capabilities. However, their direct application in industrial processes often presents challenges related to substrate scope, catalytic efficiency, operational stability, and scalability. Moreover, biological catalysts differ fundamentally from conventional chemical catalysts, requiring specialized strategies for discovery, optimization, and integration into production workflows.

Developing a functional and reliable biocatalytic system involves more than identifying an active enzyme. It requires systematic engineering at multiple levels, including substrate compatibility, catalytic mechanism optimization, gene expression, enzyme stability, reaction pathway design, and process scale-up. In many cases, successful implementation also depends on integrating molecular biology, protein engineering, systems biology, and chemical engineering principles.

Creative Enzymes has long demonstrated expertise and reliability in the development and establishment of biocatalysis systems. We provide products, services, and consultation covering every step of biocatalyst development, including the design, modification, expression, purification, production, and validation of enzymatic or microbial systems that catalyze desired reactions. By combining deep scientific knowledge with practical industrial experience, we help clients overcome technical barriers and accelerate the transition from concept to commercial application.

Industrial biocatalysis today and tomorrow Figure 1 The biocatalysis cycle. (Schmid et al., 2001)

What We Offer: Comprehensive and Customized Biocatalysis Service Portfolio

Creative Enzymes delivers a full spectrum of Biocatalysis Services, designed to support projects at all stages of development. Our services are modular, flexible, and fully customizable to meet specific technical and commercial objectives.

Our Highlighted Platforms

Biocatalyst Substrate Selection & Modification We evaluate substrate scope, binding modes, and reaction compatibility using experimental assays and computational modeling. Cofactors, additives, and reaction environments are systematically optimized to enhance catalytic efficiency and stability.	Biocatalyst Substrate Profiling	Inquiry
	Computational Modeling of Biocatalyst–Substrate Interactions
	Selection and Modification of Biocatalysis Cofactors
Biocatalyst Characterization Our characterization services include catalytic assays, kinetic parameter determination, and mechanistic studies. These data provide critical insights into enzyme behavior and guide subsequent engineering strategies.	Catalytic Activity and Kinetic Assays for Biocatalysts	Inquiry
	Mechanistic Modeling and Investigation of Biocatalysts
Biocatalyst Gene Discovery & Expression We identify novel biocatalysts through natural sampling and bioinformatics-driven gene mining. Codon optimization and expression system engineering ensure robust and scalable production of functional enzymes.	Natural Biocatalyst Sampling and Screening	Inquiry
	Codon Optimization of Biocatalysts
Biocatalyst Engineering Through rational design, directed evolution, and immobilization techniques, we tailor biocatalysts for improved activity, selectivity, stability, and operational lifetime. Multi-enzyme and whole-cell systems are developed for complex transformations.	Rational Design of Biocatalysts	Inquiry
	Continuous Directed Evolution of Biocatalysts
	Biocatalyst Immobilization and Modification
	Multi-Enzyme Cascade Reaction Systems
	Whole Cell Biocatalysts
Biocatalytic Reaction Route Development We design and optimize biocatalytic reaction pathways using metabolic flux analysis, pathway engineering, and genome editing tools, enabling efficient conversion of substrates to target products.	Metabolic Flux Analysis for Biocatalytic Systems	Inquiry
	Synthetic Pathway Design for Biocatalysis
	Genome Engineering for Biocatalytic Pathway Optimization
	Integrated Metabolic Pathway Engineering for Biocatalysis
Biocatalyst Production Process Development & Consulting Our process development services address upstream and downstream considerations, including fermentation optimization, purification strategies, and scale-up. Consulting support ensures smooth transition from laboratory to industrial production.	Biocatalyst Production Process Optimization	Inquiry
	Biocatalyst Production Process Scale-Up

Biocatalysis Services Workflow

Biocatalysis services workflow

Contact Our Team

Why Choose Us

End-to-End Capabilities

Comprehensive coverage from discovery to industrial-scale implementation.

Interdisciplinary Expertise

Integrated knowledge in molecular biology, protein engineering, metabolic engineering, and process development.

Customized and Flexible Solutions

Fully bespoke services tailored to specific technical and commercial goals.

Sustainability-Driven Design

Emphasis on environmentally friendly reagents, solvents, and processes.

Strong Academic and Industrial Partnerships

Access to cutting-edge technologies and unbiased solution selection.

Proven Reliability and Efficiency

Reduced development time, minimized by-products, and optimized yields and selectivity.

Case Studies: Representative Applications of Our Biocatalysis Services

Case 1: Cascade Catalysis in Membranes with Enzyme Immobilization for Multi-Enzymatic Conversion of CO₂ to Methanol

Co-immobilizing multiple enzymes is valuable for multi-step bioconversion, such as converting CO₂ to methanol. This study demonstrates that three enzymes—formate dehydrogenase (FDH), formaldehyde dehydrogenase (FaldDH), and alcohol dehydrogenase (ADH)—can be immobilized on flat-sheet polymeric membranes via simple, solvent-free, pressure-driven filtration. Both co-immobilization and sequential immobilization retained full enzyme activity, with similar catalytic efficiencies, although the FaldDH-catalyzed step was the cascade bottleneck. Sequential immobilization allows optimization of each step's conditions and mitigates diffusion limitations. This method provides a green, efficient approach for multi-enzymatic cascades and identifies strategies to overcome bottlenecks in CO₂-to-methanol conversion.

Evaluation of an enzymatic membrane reactor (EMR) with free and immobilized enzymes Figure 2. (a) Methanol production at different NADH concentrations with free and immobilized enzymes and (b) with recycling and reusing of free and immobilized enzymes (NADH = 10 mM). Reaction time = 30 min. Enzyme and NADH concentrations were same for free and immobilized enzyme systems. (Luo et al., 2015)

Case 2: Whole-Cell Biocatalysts for Biodiesel Fuel Production

Biodiesel fuel (BDF), composed of fatty acid alkyl esters, is a sustainable alternative to conventional diesel. While alkali catalysis dominates commercial production, enzymatic transesterification offers advantages such as fewer process steps and easier glycerol separation. The primary challenge is the high cost of lipase enzymes, limiting commercial feasibility. To address this, strategies like immobilizing fungal mycelium on biomass support particles (BSPs) and expressing lipase on yeast cell surfaces have been developed. These approaches create whole-cell biocatalysts that lower enzyme-related costs, making enzymatic biodiesel production more practical for industrial applications.

Using a yeast whole cell as a whole cell biocatalyst for biodiesel fuel production Figure 3. Schematic diagram of a yeast whole-cell biocatalyst displaying ROL via an FOL 1 anchor. In this lipase displaying system, the N-terminus of ROL including a pro-sequence Pro-ROL has been fused to the flocculation functional domain of FLO p, a lectin-like cell-wall protein of yeast. (Fukuda et al., 2008)

FAQs: Frequently Asked Questions About Biocatalysis Services

Q: What industries can benefit from biocatalysis services?

A: Biocatalysis services are broadly applicable across industries that require efficient, selective, and sustainable chemical transformations. Typical application areas include pharmaceuticals and biopharmaceuticals, fine and specialty chemicals, agrochemicals, food and feed ingredients, bio-based materials, flavors and fragrances, and biofuels. Both R&D-stage organizations and industrial manufacturers can benefit from biocatalytic solutions.
Q: Can biocatalysis replace traditional chemical catalysis?

A: In many cases, biocatalysis can partially or fully replace traditional chemical catalysis by offering higher selectivity, milder reaction conditions, and reduced environmental impact. However, biocatalysis is not always a direct substitute. Hybrid processes that combine biocatalytic and chemical steps are frequently employed to achieve optimal performance, cost efficiency, and scalability.
Q: How customizable are your biocatalysis services?

A: Our biocatalysis services are fully customizable and modular. Clients may engage us for a single technical task—such as enzyme screening or pathway optimization—or for comprehensive end-to-end development covering discovery, engineering, and process scale-up. Service scope, timelines, and deliverables are defined according to specific project goals and development stages.
Q: Do you support scale-up and industrial implementation of biocatalytic processes?

A: Yes. In addition to laboratory-scale development, we provide process optimization, scale-up support, and technical consulting for pilot and industrial-scale implementation. Our team considers upstream production, downstream processing, operational stability, and cost efficiency to facilitate smooth technology transfer to manufacturing environments.
Q: How do you ensure data confidentiality and intellectual property protection?

A: All projects are conducted under strict confidentiality agreements. Intellectual property ownership, data usage, and publication rights are clearly defined at the outset of each collaboration, ensuring alignment with client requirements and internal IP strategies.
Q: How can a biocatalysis project be initiated?

A: Projects typically begin with a technical consultation to discuss target reactions, performance requirements, timelines, and regulatory or manufacturing considerations. Based on this discussion, we propose a tailored development strategy and service plan to efficiently advance the project.

References:

Fukuda H, Hama S, Tamalampudi S, Noda H. Whole-cell biocatalysts for biodiesel fuel production. Trends in Biotechnology. 2008;26(12):668-673. doi:10.1016/j.tibtech.2008.08.001
Luo J, Meyer AS, Mateiu RV, Pinelo M. Cascade catalysis in membranes with enzyme immobilization for multi-enzymatic conversion of CO₂ to methanol. New Biotechnology. 2015;32(3):319-327. doi:10.1016/j.nbt.2015.02.006
Schmid A, Dordick JS, Hauer B, Kiener A, Wubbolts M, Witholt B. Industrial biocatalysis today and tomorrow. Nature. 2001;409(6817):258-268. doi:10.1038/35051736

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For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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