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Enzymology Assays

Creative Enzymes is a renowned service provider, supporting a majority of the enzyme assay market. Creative Enzymes endeavors to perfect all types of Enzymology Assays. We are able to perform individual tests at the most precise level, as well as design and complete a whole package of services as the solution to a thematic project.

Overview of Our Enzymology Assay Services

Enzymology assays are specialized laboratory tests designed to measure the activity, kinetics, and specificity of enzymes. These assays are fundamental in understanding how enzymes function, how they respond to inhibitors or activators, and how they behave under different physiological or industrial conditions. By quantifying reaction rates and product formation, enzymology assays provide critical data for drug development, bioprocess optimization, diagnostic test creation, and quality control in biological products.

Our expertise covers a wide range of assay formats—from high-throughput screening to detailed mechanistic studies—ensuring accurate, reproducible results that help transform biochemical questions into actionable insights. Whether for research, regulatory submissions, or process validation, our enzymology services deliver the precision and clarity your project demands.

Comprehensive Enzymology Assay Service Offerings

We provide a complete suite of enzymology assay services, from standard activity measurements to complex mechanistic studies.

Services Features Price
Activity Measurement Assays for kinases, phosphatases, proteinases, deacetylases, peptidases, esterases, and more. Kit-based and custom assays available. High-throughput screening from 200 candidates per assay. From $1,500/assay
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Development of Analysis Methods New assay design for unprecedented conditions or newly discovered enzymes. Expertise in challenging assays, including reversible reactions and coupled tests.
Enzyme Kinetics Determine all key enzyme parameters (Vmax, Kd, Km, kcat, k1, k-1) with precision.
Enzyme Catalytic Mechanisms Comprehensive mechanistic studies using crystallography, site-directed mutagenesis, reporter group labeling, kinetic isotope effect measurements, and more.

Step-by-Step Enzymology Assay Workflow

Enzymology assays workflow

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Why Choose Creative Enzymes for Enzymology Assay

Specialized Analysis and Assessment

Unmatched precision in data generation.

Comprehensive Equipment & Platforms

Full range of assay instruments for any scale.

High-Throughput Capabilities

Starting from 200 candidates per assay.

Miniaturized Assays

Enhanced sensitivity with minimal sample requirements.

Rapid Turnaround

Accurate results delivered quickly.

One-Stop Solution

From assay development to data analysis and reporting.

Enzymology Assay Case Studies and Success Stories

Case 1: High-Throughput Enzyme Activity Screening for a Drug Discovery Program

Goal

A pharmaceutical client approached Creative Enzymes to identify potent inhibitors for a novel serine protease implicated in inflammatory disorders. The objective was to develop and execute a high-throughput enzymology assay to screen over 500 small-molecule candidates for inhibitory activity under physiologically relevant conditions.

Results

  • Assay Development
    Designed a fluorometric protease activity assay using a synthetic peptide substrate labeled with a quenched fluorescent reporter.
    Optimized assay buffer composition, pH, and cofactor concentrations to achieve maximum enzyme stability and activity.
    Determined assay linearity over time and substrate concentration to avoid signal saturation during high-throughput runs.
  • High-Throughput Screening
    Implemented assay on a 384-well microplate format using an automated liquid-handling system.
    Processed 520 compound candidates in duplicate within 48 hours, including controls for baseline activity and inhibition.
    Achieved a Z'-factor of 0.82, confirming the assay's robustness and reproducibility.
  • Hit Identification
    Identified 12 compounds showing >70% inhibition at 10 μM concentration.
    Selected top 4 candidates for follow-up IC50 determination and enzyme kinetic characterization ( Ki values in the nanomolar range).

Conclusions and Discussions

The project delivered a validated, high-throughput screening platform capable of detecting potent inhibitors for the target protease. The rapid turnaround allowed the client to move promising candidates into preclinical evaluation ahead of schedule. This work demonstrated our ability to integrate assay development, automation, and kinetic analysis into a streamlined discovery pipeline.

Case 2: Custom Kinetic Analysis of a Novel Industrial Biocatalyst

Goal

An industrial biotechnology company requested a comprehensive kinetic profile for a newly engineered lipase intended for biodiesel production. The aim was to determine Vmax, Km, kcat, and catalytic efficiency under a range of solvent and temperature conditions to guide scale-up decisions.

Results

  • Assay Design
    Developed a spectrophotometric assay using p-nitrophenyl palmitate (pNPP) as the chromogenic substrate.
    Adapted assay for mixed aqueous/organic solvent systems to mimic industrial reaction environments.
  • Kinetic Measurements
    Performed reactions across substrate concentrations from 0.1 to 5 mM.
    Used non-linear regression to fit Michaelis-Menten and Lineweaver-Burk plots for parameter extraction.
    Measured Vmax = 185 ± 4 μmol·min-1·mg-1, Km = 0.42 ± 0.03 mM, kcat = 152 s-1 at 37°C in aqueous buffer.
    Identified optimal activity at 45°C in 10% (v/v) ethanol, maintaining >90% activity after 6 hours.
  • Stability and Solvent Tolerance
    Enzyme retained >80% activity after incubation at 50°C for 2 hours.
    Tolerated up to 30% methanol without significant loss in activity.

Conclusions and Discussions

The kinetic and stability data confirmed the engineered lipase's suitability for biodiesel production under semi-organic conditions. These results allowed the client to proceed confidently with pilot-scale trials, reducing R&D uncertainty and accelerating commercialization timelines. The project highlighted our capability to deliver not just standard enzymology assays but tailored protocols aligned with industrial process needs.

FAQs About Enzymology Assays

  • Q: Can I use an "existing" protocol for my enzyme assay?

    A: It is to be noted that all enzymes reach their optimum activity under different conditions, whereas in a high-throughput screening, one single reaction condition is often used. As a result, the differences in the activity levels of enzymes may be true only in the current condition and cannot be used for quantitative comparison. Another challenge that frequently troubles researchers is that detection of formation of the product or the consumption of the substrate could be strongly disturbed by other ingredients in the reaction matrix, such as inactive solutes or other enzymes. In such cases, the signal to noise ratio must be considered for accurate measurement. Proper selection of the detection method and parameters often gives solutions to such problems.
  • Q: Can you handle unusual or challenging enzyme assays?

    A: Absolutely. We specialize in developing assays for unprecedented conditions and newly discovered enzymes. From reversible reactions to coupled tests, our team uses advanced method development and a wide range of detection technologies to tackle even the most complex projects.
  • Q: How fast can I expect results?

    A: Our optimized workflows and high-throughput platforms enable rapid turnaround without compromising quality. Depending on project scope, we can deliver results for standard activity assays in as little as a few days. Complex kinetic or mechanistic studies may require more time, but you'll receive a clear timeline upfront.
  • Q: What makes your high-throughput screening different from others?

    A: Our high-throughput systems start from 200 candidates per assay, with miniaturized formats that increase sensitivity while reducing sample consumption. Combined with precise quality controls, we generate reliable, reproducible data that's ready for decision-making.
  • Q: Can you customize the assay to fit my specific research goals?

    A: Yes. Whether you need a standard kit-based assay, a modified protocol, or a fully custom method, we tailor every project to your requirements. Our one-stop service model means we can take you from initial concept to complete data package seamlessly.
  • Q: How do you ensure data accuracy and reliability?

    A: We apply stringent quality control at every step, from sample preparation to data reporting. By integrating specialized assay platforms, expert analysis, and statistical validation, we ensure your results are both accurate and reproducible.
  • Q: Do you provide support after delivering results?

    A: Definitely. Our services don't stop at sending you the data—we offer post-project consultations to help you interpret results, troubleshoot, or plan the next steps for your research or product development.

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