Abzyme Production and Purification Services

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Abzymes, or catalytic antibodies, represent a cutting-edge approach to biocatalysis, enabling the acceleration of chemical reactions that natural enzymes cannot efficiently catalyze. The production and purification of these molecules require a sophisticated integration of immunochemistry, molecular biology, and protein purification techniques. Creative Enzymes offers a comprehensive Abzyme Production and Purification Service designed to deliver high-quality, functionally active catalytic antibodies tailored to your research or industrial applications.

This service encompasses the entire workflow, from immunization using transition-state analogs (TSAs) through hybridoma or recombinant antibody generation, to purification of catalytically active abzymes. Each abzyme is produced with rigorous quality control, ensuring structural integrity, reproducibility, and catalytic functionality. Our integrated approach streamlines the generation of high-performance catalytic antibodies suitable for biochemical studies, synthetic applications, or early-stage therapeutic development.

Background on Abzyme Production and Purification

The concept of abzymes stems from the principle that antibodies can be elicited to recognize and stabilize specific chemical structures, including transition states of reactions. Unlike conventional antibodies, abzymes possess enzymatic activity, allowing them to catalyze chemical transformations with high specificity. Their applications span synthetic chemistry, diagnostics, biocatalysis, and potentially therapeutics.

Abzymes (antibody enzymes) are antibodies that possess catalytic activity

The production of abzymes begins with the rational design and chemical synthesis of stable TSAs that accurately reproduce the geometric, electronic, and stereochemical features of the target reaction's transition state. These TSAs are typically conjugated to immunogenic carriers to elicit a robust immune response. Following immunization, antibody-producing cells are isolated and screened to identify candidates exhibiting catalytic activity. Subsequent purification is essential to obtain homogeneous abzyme preparations with defined binding and catalytic properties.

Transition-state analog–based abzyme production bridges synthetic chemistry, enzymology, and immunotechnology. It enables the creation of catalytic antibodies for reactions that lack natural enzymatic counterparts, offering powerful tools for mechanistic studies, novel biocatalysis, and therapeutic research.

Abzyme Production and Purification: What We Offer

Creative Enzymes provides a full-service solution for generating and purifying catalytic antibodies, including both hybridoma-based and recombinant approaches. Our offerings include:

Services
Immunization Services	We immunize suitable animal hosts (mice, rabbits, or other models) with well-characterized TSAs or TSA–carrier conjugates, employing optimized adjuvants and immunization schedules to elicit high-affinity antibodies.	Get a quote
Hybridoma Generation	Utilizing established hybridoma techniques, we fuse antibody-producing B cells with myeloma cells to create stable cell lines secreting catalytic antibodies. High-throughput screening identifies hybridomas producing abzymes with desired activity and specificity.
Recombinant Antibody Production	For projects requiring scalability, sequence-defined recombinant expression, or humanized antibody formats, we offer mammalian or microbial expression systems capable of producing functional abzymes with high yield.
Purification of Active Abzymes	Our purification pipelines employ affinity chromatography, size-exclusion, and other techniques optimized to maintain catalytic activity, structural integrity, and homogeneity of abzyme preparations.
Functional Characterization	Initial activity screening is performed to confirm that purified abzymes retain catalytic efficiency and target specificity. Further evaluation can be coordinated with our Catalytic Activity Evaluation Service.
Comprehensive Documentation	All stages of production and purification are documented, including immunization protocols, cell line development, purification yield, and initial activity data, ensuring traceability and reproducibility.

Service Workflow

Workflow of abzyme production and purification services

Related Services Overview

Design of Transition-State Analogs (TSA): This service focuses on the rational design of high-fidelity TSAs, incorporating structural, electronic, and stereochemical features essential for inducing catalytically active antibodies. It provides the foundation for generating antibodies capable of catalyzing reactions that natural enzymes may not efficiently perform.
Chemical Synthesis of Transition-State Analogs (TSA): Once designed, TSAs must be synthesized into stable, high-purity molecules suitable for immunization. This service ensures that analogs maintain their structural and functional integrity, providing the required antigenic material for abzyme induction.
Catalytic Activity Evaluation of Abzymes: Following production and purification, the catalytic efficiency and specificity of abzymes must be characterized. This service evaluates in vitro and in vivo activity, confirming that the abzyme performs the desired chemical transformation with appropriate kinetics and selectivity.

Contact Our Team

Why Choose Us

Integrated Expertise in Abzyme Development

Our team combines immunology, enzymology, molecular biology, and protein chemistry to deliver fully functional catalytic antibodies.

Comprehensive End-to-End Services

From TSA design and synthesis to abzyme production, purification, and activity evaluation, we offer a seamless pipeline under one roof.

High-Fidelity Purification Methods

Purification protocols are tailored to preserve catalytic activity and structural integrity, ensuring consistent and reliable abzyme preparations.

Flexible Production Platforms

We support both hybridoma-based and recombinant antibody production, accommodating a wide range of research and industrial applications.

Rigorous Quality Control

Every abzyme batch is fully characterized for purity, activity, and stability, with detailed documentation provided to the client.

Customized Scientific Consultation

We provide guidance on TSA selection, immunization strategies, antibody screening, and downstream applications to maximize success.

Abzyme Production and Purification: Case Studies

Case 1: Esterase-Like Abzyme Production

Objective:

The client required a catalytic antibody capable of efficiently hydrolyzing a synthetic ester intermediate used in a specialized chemical synthesis workflow. Natural esterases exhibited limited activity and poor selectivity toward the target substrate, necessitating the development of an abzyme tailored to the reaction's transition state and operating conditions.

Approach:

A transition-state analog (TSA) was first designed to closely mimic the tetrahedral intermediate of the ester hydrolysis reaction. This TSA was conjugated to an immunogenic carrier and used to immunize mice under optimized immunization protocols. Hybridoma technology was employed to generate monoclonal antibody-producing cell lines. Following expansion, antibodies were purified using affinity chromatography and subjected to rigorous quality control, including purity, binding affinity, and structural integrity assessment. High-throughput catalytic screening assays were then performed to evaluate esterase-like activity.

Outcome:

Several monoclonal abzymes demonstrated measurable and reproducible ester hydrolysis activity, with catalytic efficiencies significantly exceeding those of non-optimized antibodies. These results confirmed the effectiveness of the production and purification strategy and provided the client with validated abzyme candidates for further development.

Case 2: Pericyclic Reaction Abzyme

Objective:

This project aimed to produce and purify an abzyme capable of catalyzing a concerted pericyclic cycloaddition reaction, a transformation not efficiently catalyzed by natural enzymes. The client required a scalable production platform yielding highly functional antibodies suitable for in vitro evaluation and downstream optimization.

Approach:

A rigid bicyclic transition-state analog was employed to elicit antibodies selective for the pericyclic transition-state geometry. Antibody genes were cloned and expressed recombinantly in mammalian expression systems to ensure proper folding and post-translational modifications. Large-scale cell culture was conducted to achieve high antibody yields, followed by multi-step purification using protein A affinity chromatography and polishing steps to ensure high purity and homogeneity. Purified abzymes were assessed for binding specificity and catalytic potential using model cycloaddition assays.

Outcome:

The purified recombinant abzymes exhibited strong affinity for the transition-state analog and demonstrated selective stabilization of the pericyclic transition state. Initial in vitro assays confirmed catalytic activity, validating the production and purification workflow and supporting further application of these abzymes in synthetic chemistry research.

Common Questions About Abzyme Production and Purification

Q: What types of TSAs are compatible with your production service?

A: We accept both in-house and client-provided TSAs, including simple molecular mimics and complex, multivalent constructs. Our team evaluates TSA stability and immunogenicity before proceeding.
Q: Can you produce both monoclonal and recombinant abzymes?

A: Yes. Hybridoma-based monoclonal production is available for small to medium-scale research needs, while recombinant expression supports high-yield, scalable antibody production.
Q: How long does abzyme production take?

A: Timelines vary based on immunization, hybridoma generation, and purification complexity, typically ranging from 3 to 6 months for hybridoma-based production and 2 to 4 months for recombinant expression.
Q: Are the purified abzymes ready for catalytic activity assays?

A: Yes. All purified abzymes are characterized for structural integrity and initial catalytic activity. Full kinetic evaluation can be performed through our catalytic activity evaluation service.
Q: Can you scale abzyme production for industrial or large-scale research use?

A: Yes. Both hybridoma and recombinant platforms can be scaled to meet larger quantity requirements while maintaining quality and activity.
Q: How is the quality of abzymes ensured?

A: Rigorous analytical testing, including purity, homogeneity, and preliminary catalytic activity assays, ensures every batch meets high standards. Detailed reports accompany each delivery.
Q: Do you provide support if initial antibody screening fails to identify active clones?

A: Yes. We offer iterative optimization strategies, including alternative TSA constructs, immunization adjustments, or additional recombinant expression to improve success rates.

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Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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