Immunohistochemical Staining with Nanozymes

Immunohistochemistry uses the principle of antigen and antibody specificity to stain specific peptides or macromolecular proteins expressed in tissue cells, so as to achieve qualitative, location or quantitative study of specific antigens in tissue or cell specimens. Immunohistochemical staining based on nanozymes has the characteristics of strong specificity, high sensitivity and accurate localization, which is conducive to the in-depth research in the field of pathology and plays an important role in modern pathological diagnosis.

Creative Enzymes possesses enriched experience in nanozymes manufacture and applications, performing various strategies to offer custom-designed nanozymes. In recent years, our scientists and expert technicians have been dedicated to customizing your program to meet your requirements. Compared to traditional immunohistochemistry, the staining method based on nanozymes is more rapid and sensitive.

Tumor Tissue Staining with Nanozymes

• Heavy-chain ferritin (HFn) nanocage specifically recognizes tumor cells via binding to overexpressed transferrin receptor1 (TfR1) in tumor cells. Iron oxide nanozymes catalyze the oxidation of color substrates in the presence of H₂O₂ to produce an intense color reaction for visualizing tumor tissues.

Fig. 1 M-HFn nanozymes as peroxidase mimics for tumor tissue staining.

• Creative Enzymes develops a magnetoferritin nanozyme (M-HFn) which is composed of a recombinant human heavy-chain ferritin protein nanocage encapsulated an iron oxide nanocore for tumor targeting and imaging. M-HFn nanozymes can identify types of cancer cells with a high specificity and sensitivity.
• Compared to traditional immunohistochemistry, the M-HFn nanozyme method is more rapid and simpler, which greatly shortens the diagnostic time and reduces the cost, thus it has significant implications for cancer diagnosis.

Cancers Staining with Nanozyme

• Creative Enzymes uses antibody Co₃O₄ nanozymes as target-specific peroxidase mimics for the immunohistochemical staining of vascular endothelial growth factor (VEGF) in tumor tissue slices.
• So far, we have developed numerous peroxidase nanozyme-based staining methods for pathological diagnosis of breast cancer, colorectal, stomach, pancreas, hepatocellular carcinoma, esophageal cancer, and bladder cancer.

Staining of Pathological Identification with Nanozyme

• M-HFn nanozymes specifically distinguish the ruptured and high-risk plaque tissues via TfR1, which is highly expressed in plaque-infiltrated macrophages and significantly associated with the increasing risk of plaque rupture.
• M-HFn nanozymes with peroxidase catalytic activity specifically distinguish high-risk plaque tissues from patients with symptomatic carotid disease, and M-HFn staining show a significant correlation with plaque vulnerability.

Fig. 2 M-HFn nanozymes specifically stain the unstable plaques.

• We recently also prepare a magnetoferritin nanozyme for the pathological identification of high-risk and ruptured atherosclerotic plaques from patients with symptomatic carotid disease.

Creative Enzymes is pleased to share our cutting-edge technology and extensive expertise in nanozymes to facilitate our clients' research and project development. We can offer high-quality customized services by adjusting protocols to meet specific requirements. If you are interested in our services, please contact us for more details or make an online inquiry.