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Development of Analysis Methods

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Assay development is the cornerstone of modern enzymology—enabling precise characterization of target enzymes, high-throughput screening for drug discovery, and quality monitoring in industrial processes. Depending on the application, assays may focus on measuring enzyme activity, detecting small molecule binding, or analyzing enzyme–protein interactions.

At Creative Enzymes, we are recognized as a global leader in enzymology services, delivering Custom-Tailored Assay Development Solutions for research, diagnostics, and manufacturing. Trusted by thousands of scientists and companies worldwide, we combine deep expertise with advanced technology to transform complex assay requirements into reliable, high-performance methods.

Custom Enzyme Assay Development Services for Accurate and Reproducible Results

Our enzyme assay method development service focuses on designing and optimizing reliable, sensitive, and reproducible protocols for measuring enzyme activity across a wide range of applications. Each method is tailored to the specific characteristics of your enzyme and intended use, ensuring results that are both scientifically rigorous and commercially relevant.

We select the most appropriate detection techniques—from spectrophotometric and fluorometric to luminescent assays—and establish optimal reaction conditions (pH, temperature, buffer composition). We also validate essential kinetic parameters, including Km, Vmax, and kcat, to provide a complete picture of enzyme performance.

Key considerations in our development process include:

  • Substrate Specificity: designing or sourcing analogs for unique enzymes
  • Signal-to-noise Optimization: minimizing interference for clearer results
  • High-throughput Adaptability: enabling scalability for screening or industrial use

We also integrate advanced tools such as microfluidic platforms, coupled assays, and computational modeling to further enhance precision, reproducibility, and adaptability.

Our Assay Development Service Offerings

Service Workflow

Enzymology assays workflow

Service Details

Custom Enzyme Assay Development

We design and tailor enzyme assays to your specific needs, optimizing detection methods and reaction conditions for sensitive, reproducible measurements. Ideal for research, drug discovery, diagnostics, and industrial applications.

Assay Optimization & Validation Services

Refine and validate existing assays for maximum accuracy, sensitivity, and reproducibility. Our systematic optimization ensures reliable results and compliance with regulatory or publication standards.

High-Throughput Adaptation

Scale up assays for automated, high-throughput screening without compromising accuracy. Compatible with 96- or 384-well formats for drug discovery, enzyme engineering, or industrial quality control.

Multiplex Enzyme Assays Development

Measure multiple enzyme activities simultaneously with minimal cross-reactivity and high sensitivity. Perfect for pathway analysis, biomarker validation, and systems biology studies.

Contact Our Team

Why Choose Creative Enzymes

Comprehensive Technique Coverage

Spectrophotometric, fluorometric, luminescent, radiometric, calorimetric, microplate-based, and advanced analytical methods (HPLC, LC–MS).

Multidisciplinary Expertise

Project teams composed of biochemists, engineers, and physicists to address every technical challenge.

Dedicated Project Manager

Single point of contact, providing clear communication and regular updates.

Pre- and Post-Project Consultation

Expert guidance to refine your assay requirements and interpret your results.

Fully Equipped

Complete library of enzymes, substrates, cofactors, and detection reagents for rapid method development.

Regulatory Compliance

Optional cGMP/cGLP-compliant processes for regulated industries.

Case Studies and Success Stories

Case 1: Optimizing Kinase IC50 Determination for a Biotech Startup

Client Challenge:

A biotech startup developing small-molecule inhibitors for neurodegenerative disease was struggling with inconsistent IC50 values across different kinase assays. Variability between assay platforms was delaying their hit-to-lead decision-making and raising concerns with investors.

Our Approach:

We designed and validated a biochemical-based kinase assay tailored to their compound series. Key steps included:

  • Establishing a robust ATP–substrate system optimized for their kinase target.
  • Implementing a luminescent ADP-detection method for high sensitivity.
  • Performing cross-validation with a cell-based phosphorylation assay to confirm biological relevance.

Outcome:

  • Reduced IC50 variability from ±30% to <10%.
  • Identified two lead compounds with reproducible potency across biochemical and cell-based systems.

Case 2: Developing a Custom High-Throughput Enzymology Assay for CRO Partner

Client Challenge:

A contract research organization (CRO) needed a high-throughput assay to profile novel protease inhibitors for oncology research. Off-the-shelf kits lacked sensitivity at low substrate concentrations, leading to false negatives in early screening.

Our Approach:

We collaborated closely to design a tailored enzymology assay:

  • Developed a fluorogenic substrate optimized for their protease target.
  • Adapted the assay to a 384-well format for high-throughput screening.
  • Introduced kinetic readouts to differentiate between reversible and irreversible inhibition.

Outcome:

  • Screening throughput increased by 4-fold without loss of accuracy.
  • Enabled discovery of three low-nanomolar inhibitors that were previously undetected.

FAQs

  • Q: I do not have an existing assay for my enzyme. Can you start from scratch?

    A: Yes. We specialize in developing new assays from the ground up, even for novel enzymes without established protocols.

  • Q: How long does assay development take?

    A: Timelines vary depending on complexity, but most projects are completed within 2–6 weeks. We provide a detailed schedule after project assessment.

  • Q: Can you adapt my assay for high-throughput screening?

    A: Absolutely. We can miniaturize and automate existing assays to fit high-throughput formats without compromising accuracy.

  • Q: Will I receive a detailed protocol?

    A: Yes. We provide a validated, ready-to-use assay protocol along with all performance data, so your team can reproduce the results independently.

  • Q: Do you handle regulatory requirements for diagnostics or pharmaceuticals?

    A: Yes. We offer cGMP/cGLP-compliant assay development for customers in regulated industries.

With Creative Enzymes, assay development is more than a technical task—it is a collaborative process that ensures your enzyme analysis methods are reliable, reproducible, and fit for purpose. Contact us today to discuss your project and receive a detailed quotation and timeline!

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For research and industrial use only, not for personal medicinal use.

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