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β-Glucosidase, thermostable, Recombinant
Cat No.
NATE-0772
Description
Beta-glucosidase is a glucosidase enzyme located in on the brush border of the small intestine that acts upon β1->4 bonds linking two glucose or glucose-substituted molecules (i.e., the disaccharide cellobiose). It is one of the cellulases, enzymes involved in the decomposition of cellulose and related polysaccharides; more specifically, an exocellulase with specificity for a variety of beta-D-glycoside substrates. It catalyzes the hydrolysis of terminal non-reducing residues in beta-D-glucosides with release of glucose.
Abbr
β-Glucosidase, Recombinant
Alias
β-Glucosidase
Source
E. coli
Applications
β-Glucosidase is a lysosomal enzyme which breaks β1->4 bonds that link oligosaccharides. β-Glucosidase is used to study Gaucher disease and potential treatments such as enzyme replacement therapy. β-Glucocerebrosidase is an enzyme with glucosylceramidase activity (EC 3.2.1.45) that is needed to cleave, by hydrolysis, the beta-glucosidic linkage of the chemical glucocerebroside, an intermediate in glycolipid metabolism. It is localized in the lysosome and has a molecular weight of 59700 Daltons.
Package
expressed in E. coli
Form
liquid, Supplied as a solution in 50 mM Tris-HCl, pH 7.5, 100 mM NaCl, and 25% glycerol.
Activity
> 24 units/mg protein
CAS No.
9001-22-3
Molecular Weight
mol wt 53 kDa
Purity
> 90% (SDS-PAGE) 19-21 mg protein/mL (UV)
Unit Definition
One unit will produce 1 μmole of o-nitrophenol from 2-nitrophenyl β-D-glucopyranoside per minute at pH 5.8 at 70°C.
Storage
2-8°C
Synonyms
9001-22-3; β-Glucosidase, thermostable; gentiobiase; cellobiase; emulsin; elaterase; aryl-beta-glucosidase; beta-D-glucosidase; beta-glucoside glucohydrolase; arbutinase; amygdalinase; p-nitrophenyl beta-glucosidase; primeverosidase, amygdalase; linamarase; salicilinase; beta-1,6-glucosidase; β-Glucocerebrosidase; acid β-glucosidase
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Download Datasheet"β-Glucosidase" Total Products Page
| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| NATE-1942 | ∆-4,5–Glycuronidase from Flavobacterium heparinum | EC 3.2.1- | Flavobacterium heparinum (ATCC 13125) | Inquiry | |
| DIA-195 | Native Sweet almond β-Glucosidase | EC 3.2.1.21 | 9001-22-3 | Sweet almond | Inquiry |
| NATE-0306 | Native Aspergillus niger Glucosidase | EC 3.2.1- | 9001-22-3 | Aspergillus niger | Inquiry |
| NATE-0769 | Native Almonds β-Glucosidase | EC 3.2.1.31 | 9001-22-3 | Almonds | Inquiry |