α,γ-Homocysteinase from Trichomonas vaginalis, Recombinant

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Cat No.

NATE-1640

Description

Recombinant Homocysteine α,γ-lyase is a pyridoxal-5’-phosphate dependent enzyme. It is a mutant of homocysteinase from Trichomonas vaginalis encoded by mgl1 gene, containing three point mutations, such as; Phe47Leu, Asp172Glu, Ser308Tyr. The enzyme can metabolize homocysteine into α-keto butyrate, hydrogen sulfide and ammonia.

Abbr

rHCYase, Recombinant (T. vaginalis)

Source

E. coli and fused to His-tag at N-terminus

Species

Trichomonas vaginalis

Form

Lyophilized

Activity

> 5 mU/mg

Molecular Weight

43 kDa

Purity

> 95% by SDS-PAGE

Unit Definition

One Unit enzyme converts 1 µmole of homocysteine into hydrogen sulfide, per minute at 25°C and pH 8.1 in the presence of pyridoxal phosphate.

Storage

Reconstituted enzyme can be stored in working aliquots at -20°C and use within 3 months. Avoid repeated freeze-thaw cycles.

Synonyms

α,γ-Homocysteinase; Methionine gamma-lyase; mgl1

Reconstitution

Reconstitute in 40 mM Sodium phosphate buffer, pH 8.1.

Formulation

In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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