β-Mannanase from Clostridium thermocellum, Recombinant

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Cat No.

NATE-1184

Description

Mannan endo-1,4-beta-mannosidase is an enzyme with system name 4-beta-D-mannan mannanohydrolase. This enzyme catalyses the following chemical reaction: Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

Abbr

β-Mannanase, Recombinant (Clostridium thermocellum)

Source

Clostridium thermocellum YS

Form

Supplied in 35 mM HEPES buffer, pH 7.5, containing 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, 0.02 % (w/v) sodium azide and 25% (v/v) glycerol.

Enzyme Commission Number

EC 3.2.1.78

Activity

1500 U/mg

CAS_No

37288-54-3

Molecular Weight

42700 Da

Purity

> 95 % as judged by SDS-PAGE

Concentration

1500 U/ml

Unit Definition

One unit is defined as the amount of enzyme required to release 1μmol of mannose-reducing-sugar equivalents per minute from galactomannan in phosphate-Citrate (PC) buffer (50 mM K2HPO4, 12 mM citric acid, pH 6.5) at 60 °C.

Optimum pH

6.5 (stable from 6 - 8)

Optimum temperature

65°C (stable up to 70°C)

Storage

Store at -20°C (shipped at room temperature)

Synonyms

endo-1,4-β-mannanase; endo-β-1,4-mannase; β-mannanase B; β-1, 4-mannan 4-mannanohydrolase; endo-β-mannanase; β-D-mannanase; mannan endo-1,4-β-mannosidase; 1,4-β-D-mannan mannanohydrolase; EC 3.2.1.78

"endo-1,4 β-Mannanase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
BMF-001	β-Mannanase (food grade)	EC 3.2.1.78	37288-54-3		Inquiry
BMCJ-001	β-Mannanase 26A from Cellvibrio japonicus, Recombinant	EC 3.2.1.78	37288-54-3	E. coli	Inquiry
NATE-1457	Mannanase 5A from Ruminococcus flavefaciens, Recombinant	EC 3.2.1.78	37288-54-3	E. coli	Inquiry
NATE-1458	β-Mannanase 5A from Thermotoga petrophila, Recombinant	EC 3.2.1.78	37288-54-3	E. coli	Inquiry

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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