2,4-dinitrotoluene dioxygenase

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Cat No.

EXWM-0691

Description

This enzyme, characterized from the bacterium Burkholderia sp. strain DNT, is a member of the naphthalene family of bacterial Rieske non-heme iron dioxygenases. It comprises a multicomponent system, containing a Rieske [2Fe-2S] ferredoxin, an NADH-dependent flavoprotein reductase (EC 1.18.1.3, ferredoxin-NAD+ reductase), and an α3β3 oxygenase. The enzyme forms a cis-dihydroxylated product that spontaneously rearranges to form a catechol with accompanying release of nitrite. It does not act on nitrobenzene. cf. EC 1.14.12.23, nitroarene dioxygenase.

Form

Liquid or lyophilized powder

Enzyme Commission Number

EC 1.14.12.24

Storage

Store it at +4 ºC for short term. For long term storage, store it at -20 ºC～-80 ºC.

Synonyms

dntA (gene name)

Reaction

2,4-dinitrotoluene + NADH + O2 = 4-methyl-5-nitrocatechol + nitrite + NAD+

Notes

This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.

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Project Description:

For research and industrial use only, not for personal medicinal use.

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