3α-Hydroxysteroid Dehydrogenase from B. choshinensis, Recombinant

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Cat No.

DIA-413

Description

In enzymology, a 3alpha-hydroxysteroid dehydrogenase (B-specific) (EC 1.1.1.50) is an enzyme that catalyzes the chemical reaction: androsterone + NAD(P)+ ↔ 5alpha-androstane-3,17-dione + NAD(P)H + H+. The 3 substRates of this enzyme are androsterone, NAD+, and NADP+, whereas its 4 products are 5alpha-androstane-3,17-dione, NADH, NADPH, and H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor, more specifically it is part of the group of hydroxysteroid dehydrogenases.

Abbr

3α-HSD, Recombinant (B. choshinensis)

Source

B. choshinensis

Species

B. choshinensis

Appearance

White lyophilizate

Enzyme Commission Number

EC 1.1.1.50

Activity

> 30 U/mg lyophilizate

CAS_No

9028-56-2

Molecular Weight

ca. 41 kDa

pH Stability

6.0–10.0

Michaelis Constant

2.4 x 10^-5 M (androsterone) 3.0 x 10^-6 M (NAD)

Structure

2 subunits of 25 kDa (SDS-PAGE)

Unit Definition

One unit (U) is defined as the amount of enzyme which produces 1 μmol of NADH per min at 25°C and pH 8.9.

Optimum pH

Optimum temperature

50–60°C

Thermal stability

below 45°C

Stability

stable at 37°C for at least four weeks

Storage

at -20°C

Stabilizers

Trehalose

Synonyms

hydroxyprostaglandin dehydrogenase; 3α-hydroxysteroid oxidoreductase; sterognost 3α; 3α-hydroxysteroid dehydrogenase (B-specific); 3α-hydroxysteroid 3-dehydrogenase (B-specific); 3α-hydroxysteroid:NAD(P)+ 3-oxidoreductase (B-specific); EC 1.1.1.50

"3α-Hydroxysteroid Dehydrogenase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-0007	Native Pseudomonas testosteroni 3α-Hydroxysteroid Dehydrogenase	EC 1.1.1.50	9028-56-2	Pseudomonas testosteroni	Inquiry
NATE-1138	3α-Hydroxysteroid Dehydrogenase, Recombinant	EC 1.1.1.50	9028-56-2		Inquiry
EXWM-0335	3α-hydroxysteroid 3-dehydrogenase (Si-specific)	EC 1.1.1.50	9028-56-2		Inquiry

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