3,3',5,5'-Tetramethylbenzidine

Cat No.

CSUB-0445

Applications

3,3',5,5'-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.

Enzyme Commission Number

259-364-6

CAS No.

54827-17-7

Molecular Weight

240.34

Storage

2-8°C

Synonyms

BM blue; Sure Blue TMB; TMB Blotting Plus; TMB substrate; TMB

MDL Number

MFCD00007748

PubChem Substance ID

24900090

mp

168-171 °C(lit.)

Beilstein Registry Number

2808541

Substrates

Peroxidase Substrates

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