Applications
X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.
Enzyme Commission Number
230-640-8
Synonyms
5-Bromo-4-chloro-3-indolyl β-D-galactoside; BCIG; X-Gal
Molecular Formula
C14H15BrClNO6
PubChem Substance ID
24891761
Beilstein Registry Number
1402009
Substrates
Galactosidase, beta