5-Bromo-4-chloro-3-indolyl phosphate disodium salt

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Cat No.

CSUB-0035

Applications

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.

CAS_No

102185-33-1

Molecular Weight

370.43

Storage

-20°C

Synonyms

BCIP; X-phosphate disodium salt

MDL Number

MFCD00036757

Solubility

H2O: soluble 20 mg/mL

PubChem Substance ID

24891897

Substrates

Alkaline Phosphatase

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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