5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt

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Cat No.

CSUB-0050

Applications

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.

Enzyme Commission Number

229-506-1

CAS_No

6578-06-9

Molecular Weight

433.62

Storage

2-8°C

Synonyms

BCIP p-toluidine salt; X-phosphate p-toluidine salt

Molecular Formula

C8H6BrClNO4P · C7H9N

MDL Number

MFCD00040636

PubChem Substance ID

24850179

Substrates

Alkaline Phosphatase

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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