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Aldolase A from Human, Recombinant

Cat No.
NATE-1663
Description
Fructose bisphosphate aldolase A, also known as Aldolase A is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. It is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Deficiency has been associated with myopathy and hemolytic anemia. Recombinant human Aldolase A, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Abbr
Aldolase A, Recombinant (Human)
Source
E. coli
Species
Human
Form
Liquid
Enzyme Commission Number
EC 4.1.2.13
Activity
> 1.5 units/mg
Molecular Weight
41.5 kDa (384 aa, 1-364 aa + His Tag), confirmed by MALDI-TOF.
Purity
> 95% by SDS-PAGE
Unit Definition
One unit will convert 1.0 µmole of fructose 1, 6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3- phosphate per minute at pH 7.5 at 25°C.
Storage
Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Synonyms
Fructose bisphosphate Aldolase A; ALDOA; ALDA; GSD12

"Aldolase" Total Products Page

Catalog Product Name EC No. CAS No. Source Price
NATE-0048 Native Rabbit Aldolase EC 4.1.2.13 9024-52-6 Rabbit muscle Inquiry
NATE-0049 Native Spinach Aldolase EC 4.1.2.13 9024-52-6 Spinach Inquiry
NATE-1152 Native Fructose-bisphosphate aldolase from Thermophillic bacteria EC 4.1.2.13 9024-52-6 Thermophillic bacteria Inquiry
EXWM-4853 fructose-bisphosphate aldolase EC 4.1.2.13 9024-52-6 Inquiry
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