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Chemically modified Cholesterol Oxidase from Brevibacterium species

Cat No.
DIA-284
Description
Oxidoreductase that catalyzes the interconversion of cholesterol to cholest-4-en-3-one. Rely on the proven diagnostic quality of this product.
Abbr
Chemically modified CHOD, Recombinant (Brevibacterium sp.)
Alias
CHOD
Source
Brevibacterium species
Applications
Use Cholesterol Oxidase in diagnostic tests for the determination of cholesterol in combination with Cholesterol Esterase.
Appearance
Yellow lyophilizate
Activity
10-20 U/mg lyophilizate
Contaminants
Catalase: <6.0 Glucose oxidase: <0.01 "NADH oxidase": <0.01 Uricase: <0.01
Molecular Weight
60 kD (native and SDS)
Isoelectric point
~5.0
pH Stability
5.0-10.0
Michaelis Constant
Cholesterol: 1 x 10-4 mol/l
Specificity
cholesterol 100% pregnenolon 52% stigmasterol 17% dehydroandrosterone 0.5% androsterone 0% estradiol 0% cholate 0%
Optimum pH
5.5-8.0
Activators
Non ionic detergents
Thermal stability
Up to +55°C
Stability
At -15 to -25°C within specification range for 12 months. Store dry. Protect from light.
Storage
No decrease in activity over 6 weeks at +35°C.
Inhibitors
Hg2+, ZnCl2, SDS
Synonyms
Cholesterol-O2 oxidoreductase; 3 beta-Hydroxy steroid oxidoreductase; 3β-hydroxysteroid: oxygen oxidoreductase; cholesterol: oxygen oxidoreductase; cholesterol oxidase
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