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Creatininase from E. coli, Recombinant

Cat No.
NATE-1242
Description
Creatininase from Pseudomonas sp. is a homohexameric enzyme with a molecular mass of 28.4 kDa per subunit. It is a cyclic amidohydrolase catalysing the reversible conversion of creatinine to creatine. Each monomer contains a binuclear zinc centre near the C termini of the β-strands and the N termini of the main α-helices. These zinc ions indicate the location of the active site.
Abbr
Creatininase, Recombinant (E. coli)
Source
E. coli
Species
E. coli
Appearance
White lyophilizate
Enzyme Commission Number
EC 3.5.2.10
Activity
> 500 U/mg lyophilizate
CAS No.
9025-13-2
Contaminants
catalase < 1.0%
Molecular Weight
ca. 170 kDa
Isoelectric point
4.8
pH Stability
7.0–11.0
Michaelis Constant
3.4 x 10^-2 M (creatinine) 4.3 x 10^-2 M (creatine)
Structure
6 subunits of 28 kDa (SDS-PAGE)
Unit Definition
One unit (U) is defined as the amount of enzyme which produces 1 μmol of creatine per min at 37°C and pH 6.8.
Optimum pH
6.5–7.0
Optimum temperature
60–65°C
Activators
Mg2+, Mn2+
Thermal stability
below 60°C
Storage
at -20°C
Inhibitors
Hg2+
Synonyms
EC 3.5.2.10, creatinine hydrolase; Creatininase; 9025-13-2
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogDIA-130
EC No.EC 3.5.2.10
CAS No.9025-13-2
SourcePseudomonas sp.
CatalogNATE-0163
EC No.EC 3.5.2.10
CAS No.9025-13-2
Sourcemicroorganisms
CatalogEXWM-4493
ProductNamecreatininase
EC No.EC 3.5.2.10
CAS No.9025-13-2
Source
Realted Protocols
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