Products

Enzymes for Research, Diagnostic and Industrial Use

Endoproteinase GluC from Staphylococcus aureus, Recombinant

Cat No.
NATE-1270
Description
Protease S. aureus V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium, the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27 kDa daltons and optimum pH's of 4.0 and 7.8 with hemoglobin as the substrate. Protease S. aureus V8 is inhibited by diisopropylfluorophosphate and monovalent anions such as F-, Cl-, CH3COO-and NO3. Enzyme activity is determined by the casein digestion assay described by Drapeau.
Abbr
V8 Protease, Recombinant (Staphylococcus aureus)
Source
Bacillus subtilis
Species
Staphylococcus aureus
Applications
• Digestion of proteins for proteomic analysis by Mass Spectrometry • Protein and peptide identification
Product Overview
Endoproteinase GluC (Staphylococcus aureus Protease V8) is a serine proteinase which selectively cleaves peptide bonds C-terminal to glutamic acid residues. Endoproteinase GluC also cleaves at aspartic acid residues at a rate 100-300 times slower than at glutamic acid residues.
Form
Supplied freeze-dried from a Tris-HCl and sodium chloride buffer.
Activity
38.3 μmol/min/mg
CAS No.
66676-43-5
Molecular Weight
29849 daltons
Storage
at -20°C
Synonyms
EC 3.4.21.19; Staph aureus V8 Protease; Protease, Staph aureus (Endoproteinase Glu-C); Glutamyl endopeptidase; V8 proteinase, endoproteinase Glu-C; staphylococcal serine proteinase
Download Datasheet:


“V8 Protease” Total Products Page

Inquiry
Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-0730
EC No.EC 3.4.21.19
CAS No.137010-42-5
SourceStaph aureus V8
CatalogEXWM-4118
EC No.EC 3.4.21.19
CAS No.137010-42-5
Source
Online Inquiry
Name:
*Phone:
*E-mail Address:
Country:
Company/Institution:
*Products or Services Interested:
Quantity:
Project Description:
Our Products are Not Intended for Private Therapeutic Use!
*Verification Code:
Please input "enzymes"(case insensitive) as verification code.

Welcome! For price inquiries, please feel free to contact us through the form below through the form on the left side. We will get back to you as soon as possible.

Sitemap  Privacy Policy
Copyright ©2011 - 2017 Creative Enzymes.
Contact Us 45-1 Ramsey Road, Shirley, NY 11967, USA
Email: info@creative-enzymes.com
Tel: 1-631-562-8517 1-631-448-7888
Fax: 1-631-938-8127
Distributors To view the contact information for a specific location, select the desired country: