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Glucokinase from Human, recombinant

Cat No.
NATE-1686
Description
Glucose is phosphorylated to glucose-6-phosphate by glucokinases. This gene is alternatively spliced to generate three different forms of the enzyme; one found in the pancreas and two found in the liver. The main function of this gene is to regulate carbohydrate metabolism. Recombinant human pancreatic Glucokinase has a C-terminal FLAG tag and has 470 amino acid residues. It can be useful for studies including enzyme kinetics, activator screening and kinase selectivity.
Abbr
GCK, Recombinant (Human)
Source
E. coli
Species
Human pancreatic
Form
Liquid
Enzyme Commission Number
EC 2.7.1.2
Activity
303 pmol/min/µg
CAS No.
9001-36-9
Molecular Weight
53.1 kDa
Purity
> 80% by SDS-PAGE
Concentration
1 mg/ml
Unit Definition
One unit is defined as the amount of enzyme that will convert 1 pmol of NADP to NADPH at 30°C. Assay conditions: 25 mM HEPES, pH 7.5, 2 mM MgCl2, 1.0 mM DTT, 0.5 mM NADP, 2.0 mM ATP, 25 mM glucose, 100 µg/ml BSA, 20 units/ml glucose 6-phosphate dehydrogenase, and 10 nM human pancreatic glucokinase at 30°C for 30 min.
Storage
Store at -80°C. Avoid repeated freezing and thawing cycles.
Synonyms
EC 2.7.1.2; glucokinase; glucokinase (phosphorylating); 9001-36-9; GCK; FGQTL3; GK; GLK; HHF3; HK4; HKIV; HXKP; LGLK; MODY2; Hexokinase type IV; HK IV; Hexokinase-4; Hexokinase-D
Formulation
0.5 mg/ml solution in 25 mM Na2HPO4 and 500 mM NaCl (pH 7.0) with 50% glycerol.
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-1687
EC No.EC 2.7.1.2
CAS No.9001-36-9
SourceE. coli
CatalogNATE-1696
EC No.EC 2.7.1.2
CAS No.9001-36-9
SourceE. coli
CatalogNATE-1697
EC No.EC 2.7.1.2
CAS No.9001-36-9
SourceE. coli
CatalogNATE-1903
EC No.EC 2.7.1.2
CAS No.9001-36-9
SourceZymomonas mobilis
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Realted Service
Realted Protocols
glucokinase -Enzymatic Assay Protocol
nad-kinase -Enzymatic Assay Protocol
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