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Immobilized Pepsin (Agarose Resin)

Cat No.
NATE-1866
Description
Immobilized Pepsin allows efficient generation of F(ab´)2 from IgG. Pepsin is a nonspecific endopeptidase that is active only at acid pH and irreversibly denatured at neutral or alkaline pH. Immobilized Pepsin is advantageous because the digestion can be immediately stopped by simply removing the gel from the IgG. Digestion by pepsin normally produces a F(ab´)2 fragment and numerous small peptides of the Fc portion. The resulting F(ab´)2 fragment is composed of two disulfide-connected Fab units. The Fc fragment is extensively degraded, and its small fragments can be separated from F(ab´)2 by dialysis, gel filtration or ion exchange chromatography.
Package
5mL settled gel, contains sufficient material to generate F(ab´)2 fragments from up to 20 samples containing up to 10mg of IgG.
Form
50% slurry containing 50% glycerol in 0.1M sodium acetate; pH 4.5, and 0.05% sodium azide as a preservative.
Enzyme Commission Number
EC 3.4.23.1
Storage
Upon receipt store product at 4-8°C. Product is shipped at ambient temperature.
Synonyms
Immobilized Pepsin
Refractive Index
1.61 (Predicted)
Density
~1.2 g/cm3 (Predicted)
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