Products

Enzymes for Research, Diagnostic and Industrial Use

Immobilized TLCK-Chymotrypsin on F7m

Cat No.
NATE-1769
Description
α-chymotrypsin hydrolyzes peptides, amides, and esters preferentially at the carboxyl groups of hydrophobic amino acids (L-tyrosine, L-phenylalanine, and L-tryptophan but also bonds of leucyl, methionyl, asparaginyl, and glutamyl residues).
F7m: 1. 0 mg α-chymotrypsin per CR-column immobilized on polyvinyl.
55 units immobilized per CR-column
Nr. 5 Storage buffer: 50 mM Tris/HCl, pH 7. 5
Nr. 5 Reaction buffer: 50 mM Tris/HCl, pH 7. 5; also active in 0.1% SDS
Nr. 6 Washing buffer: 50 mM Tris/HCl, 1 M NaCl, pH 7. 5
Source
Bovine pancreas
Enzyme Commission Number
EC 3. 4. 21. 1
Storage
4 °C
Synonyms
EC 3. 4. 21. 1; α-Chymotrypsin; chymotrypsins A and B; alpha-chymar ophth; avazyme; chymar; chymotest; enzeon; quimar; quimotrase; alpha-chymar; alpha-chymotrypsin A; alpha-chymotrypsin; Chymotrypsin
Protocol
1. Dilute delivered buffers (at least 2 ml each) with sterile doubly distilled water.
For 1 application you need:
0.25 ml 10x reaction buffer and 2. 25 ml doubly distilled water
0.4 ml 5x washing buffer and 1. 6 ml doubly distilled water
0.2 ml 10x storage buffer and 1. 8 ml doubly distilled water
The substrate should be in reaction buffer
2. Equilibrate the CR-column with 2 ml reaction buffer.
Fill 2 ml reaction buffer into a syringe, let the reaction buffer run through the column by gravity to the upper filter. In case the buffer runs very slowly, apply pressure by a syringe.
3. Load substrate solution in reaction buffer.
Small volumes (< 80 µl): spin the CR-column 5 seconds in a benchtop centrifuge (2000 rpm are sufficient). Let the substrate solution enter the matrix material.
Larger volumes: Let the substrate solution run through the column.
Flow-rate: up to 80 µl/minute
Keep the substrate in the column for about 1 minute at room temperature. Higher turn-over is obtained when the substrate is applied to the column again or incubated for longer times.
4. Elute the product solution.
Small volumes (< 80 µl): Elute the product with 500 µl reaction buffer.
Larger volumes: Let the substrate run through the column and elute the residual product solution with 500 µl reaction buffer.
It does not harm the columns if they run dry.
5. Wash the column with 2 ml washing buffer.
6. Equilibrate the column with 2 ml storage buffer.
Store the column at 4°C.
Never freeze a CR-column!
Download Datasheet:



Inquiry
Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-1754
EC No.EC 3. 4. 21. 1
CAS No.
SourceBovine pancreas
CatalogNATE-1770
EC No.EC 3. 4. 21. 1
CAS No.
SourceBovine pancreas
CatalogNATE-0746
EC No.EC 3.4.21.1
CAS No.9004-07-3
SourceBovine Pancreas
CatalogNATE-0747
EC No.EC 3.4.21.1
CAS No.9004-07-3
SourceHuman pancreas
+ See More >>
Online Inquiry
Name:
*Phone:
*E-mail Address:
Country:
Company/Institution:
*Products or Services Interested:
Quantity:
Project Description:
Our Products are Not Intended for Private Therapeutic Use!
*Verification Code:
Please input "enzymes"(case insensitive) as verification code.

Welcome! For price inquiries, please feel free to contact us through the form below through the form on the left side. We will get back to you as soon as possible.

Sitemap  Privacy Policy
Copyright ©2011 - 2017 Creative Enzymes.
Contact Us 45-1 Ramsey Road, Shirley, NY 11967, USA
Email: info@creative-enzymes.com
Tel: 1-631-562-8517 1-631-448-7888
Fax: 1-631-938-8127
Distributors To view the contact information for a specific location, select the desired country: