Applications
Electron acceptor for the colorimetric assay of various dehydrogenasesIodonitrotetrazolium (INT) is a tetrazolium dye precursor that forms a purple formazan dye on reduction and has been used in a variety of applications. It is considered to have higher reactivity than some tetrazolium compounds, at least with respect to succinate dehydrogenase, with optimal results obtained using a concentration of 0.8 mM INT. INT is used as an electron acceptor for the colorimetric assays of: lactate dehydrogenase, xanthine dehydrogenase, lactyl-CoA dehydrogenase, succinate dehydrogenase, BBM II ketolisomerase, histidinol dehydrogenase and diverse other hydrolases.
Enzyme Commission Number
205-676-2
Synonyms
2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride; p-Iodonitrotetrazolium Violet; INT
Molecular Formula
C19H13ClIN5O2
Solubility
methanol: water (1:1): soluble 50 mg/mL, very faintly turbid, very deep yellow (hot)
PubChem Substance ID
24881028
Beilstein Registry Number
4093224
Substrates
Peroxidase Substrates
