Iodonitrotetrazolium chloride

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Cat No.

CSUB-0446

Applications

Electron acceptor for the colorimetric assay of various dehydrogenasesIodonitrotetrazolium (INT) is a tetrazolium dye precursor that forms a purple formazan dye on reduction and has been used in a variety of applications. It is considered to have higher reactivity than some tetrazolium compounds, at least with respect to succinate dehydrogenase, with optimal results obtained using a concentration of 0.8 mM INT. INT is used as an electron acceptor for the colorimetric assays of: lactate dehydrogenase, xanthine dehydrogenase, lactyl-CoA dehydrogenase, succinate dehydrogenase, BBM II ketolisomerase, histidinol dehydrogenase and diverse other hydrolases.

Enzyme Commission Number

205-676-2

CAS_No

146-68-9

Molecular Weight

505.70

Synonyms

2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride; p-Iodonitrotetrazolium Violet; INT

Molecular Formula

C19H13ClIN5O2

MDL Number

MFCD00011961

Solubility

methanol: water (1:1): soluble 50 mg/mL, very faintly turbid, very deep yellow (hot)

PubChem Substance ID

24881028

240 °C (dec.)(lit.)

Beilstein Registry Number

4093224

Substrates

Peroxidase Substrates

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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