Products

Enzymes for Research, Diagnostic and Industrial Use

Luciferase from Photinus pyralis (firefly), Recombinant

Cat No.
NATE-0424
Description
Firefly luciferase is an enzyme that catalyzes production of light from luciferin in the presence of Mg2+-ATP and oxygen. The reaction of this enzyme with luciferin, ATP, and O2 results in the emission of light. Luciferase activity can be inhibited by general anesthetics including isoflurane and ketamine/medetomidine thereby affecting the sensitivity of bioluminescence imaging.
Abbr
Luciferase, Recombinant (Photinus pyralis (firefly))
Alias
Luciferase
Source
E. coli
Species
Photinus pyralis (firefly)
Applications
The reaction of this enzyme with luciferin, ATP, and O2 results in the emission of light. Luciferase can be used to detect trace amounts of ATP. Firefly luciferase is also one of the most commonly utilized reporter genes for the study of gene expression. The bioluminescent reaction catalyzed by luciferase is one of the most sensitive analytical tools for measuring gene expression. < or equal to one femtomole of ATP can be detected using 0.2 μg of luciferase.
Form
Type I, Supplied as a solution in 50 mM Tris HCl pH 8.0, 1 mM dithiothreitol, 1 mM EDTA, and 10% (v/v) glycerol; Type II, Lyophilized powder approximately 20% protein; balance is primarily NaCl, HEPES buffer salts, and carbohydrate..
Enzyme Commission Number
EC 1.13.12.7
Activity
Type I , > 5 ,000 ,000 units/mg protein; Type II, 15-30×106 light units/mg protein.
CAS No.
61970-00-1
Molecular Weight
mol wt 120 kDa (two subunits)
Unit Definition
One light unit produces a biometer peak height equivalent to 0.02 μCi of 14C in PPO/POPOP cocktail. Light units measured in 50 μl assay mixture containing 5 pmol ATP and 7.5 nmol luciferin in Tris-glycine buffer, pH 7.6, at 25°C.
Storage
−70°C
Synonyms
Photinus-luciferin 4-monooxygenase (ATP-hydrolysing); firefly luciferase; luciferase (firefly luciferin); Photinus luciferin 4-monooxygenase (adenosine triphosphate-hydrolyzing); firefly luciferin luciferase; Photinus pyralis luciferase; EC 1.13.12.7; 61970-00-1
Download Datasheet:


“Luciferase” Total Products Page

Inquiry
Related Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-0422
EC No.EC 1.13.12.7
CAS No.9014-00-0
SourcePhotinus pyralis (firefly)
CatalogNATE-0423
EC No.EC 1.13.12.7
CAS No.9014-00-0
SourceVibrio fischeri (Photobacterium f)
CatalogNATE-1253
EC No.EC 1.13.12.7
CAS No.
SourceE. coli
CatalogNATE-1254
EC No.EC 1.13.12.7
CAS No.
SourceE. coli
+ See More >>
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogCSUB-0354
ProductNameD(-)-Luciferin
EC No.
CAS No.2591-17-5
Source
CatalogCSUB-0355
ProductNameD-Luciferin
EC No.219-981-3
CAS No.2591-17-5
Source
CatalogCSUB-0356
EC No.
CAS No.103404-75-7
Source
CatalogCSUB-0357
ProductNameD-Luciferin
EC No.219-981-3
CAS No.2591-17-5
Source
+ See More >>
Related Services
Online Inquiry
Name:
*Phone:
*E-mail Address:
Country:
Company/Institution:
*Products or Services Interested:
Quantity:
Project Description:
Our Products are Not Intended for Private Therapeutic Use!
*Verification Code:
Please input "enzymes"(case insensitive) as verification code.

Welcome! For price inquiries, please feel free to contact us through the form below through the form on the left side. We will get back to you as soon as possible.

Sitemap  Privacy Policy
Copyright ©2011 - 2017 Creative Enzymes.
Contact Us 45-1 Ramsey Road, Shirley, NY 11967, USA
Email: info@creative-enzymes.com
Tel: 1-631-562-8517 1-631-448-7888
Fax: 1-631-938-8127
Distributors To view the contact information for a specific location, select the desired country: