Products

Enzymes for Research, Diagnostic and Industrial Use

Native Actinobacillus sp. Creatinase

Cat No.
NATE-0160
Description
In enzymology, a creatinase (EC 3.5.3.3) is an enzyme that catalyzes the chemical reaction:creatine + H2O↔ sarcosine + urea. Thus, the two substrates of this enzyme are creatine and H2O, whereas its two products are sarcosine and urea. This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amidines. Creatinase accelerates the conversion reaction of creatine and water molecule to sarcosine and urea. It always acts in homodimer state and is induced by choline chloride.
Abbr
Creatinase, Native (Actinobacillus sp.)
Alias
creatinase
Source
Actinobacillus sp.
Applications
Creatinase mixed with sarcosine oxidase may be used to determine the level of creatine in different pH, temperature, enzyme ratio, and buffer concentration. It may also be used to determine the plasma creatinine level by using a centrifugal analyser.
Form
Lyophilized powder containing sugars and EDTA as stabilizers
Enzyme Commission Number
EC 3.5.3.3
Activity
20-40 units/mg protein
CAS No.
37340-58-2
Molecular Weight
mol wt ~100 kDa
Isoelectric point
4.6 ± 0.1
pH Stability
pH 5.5 − 9.0 (25°C, 16hr)
Michaelis Constant
1.9 x 10‾2M (Creatine)
Structure
2 subunits per mole of enzyme
Unit Definition
One unit will hydrolyze 1.0 μmole of creatine to urea and sarcosine per min at pH 7.5 at 37°C.
Optimum pH
8
Optimum temperature
40°C
Thermal stability
Below 50°C (pH 7.5, 30 min)
Storage
−20°C
Inhibitors
Cu++, Hg++, Ag+
Synonyms
Creatine amidinohydrolase; creatinase; 37340-58-2; EC 3.5.3.3
Download Datasheet:


“Creatinase” Total Products Page

Inquiry
Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogDIA-141
EC No.EC 3.5.3.3
CAS No.37340-58-2
SourceMicroorganism
CatalogDIA-185
EC No.EC 3.5.3.3
CAS No.37340-58-2
SourceMicroorganism
CatalogNATE-0161
EC No.EC 3.5.3.3
CAS No.37340-58-2
SourceFlavobacterium sp.
CatalogNATE-0162
EC No.EC 3.5.3.3
CAS No.37340-58-2
SourceE. coli
+ See More >>
Realted Protocols
CREATINASE -Enzymatic Assay Protocol
Online Inquiry
Name:
*Phone:
*E-mail Address:
Country:
Company/Institution:
*Products or Services Interested:
Quantity:
Project Description:
Our Products are Not Intended for Private Therapeutic Use!
*Verification Code:
Please input "enzymes"(case insensitive) as verification code.

Welcome! For price inquiries, please feel free to contact us through the form below through the form on the left side. We will get back to you as soon as possible.

Sitemap  Privacy Policy
Copyright ©2011 - 2017 Creative Enzymes.
Contact Us 45-1 Ramsey Road, Shirley, NY 11967, USA
Email: info@creative-enzymes.com
Tel: 1-631-562-8517 1-631-448-7888
Fax: 1-631-938-8127
Distributors To view the contact information for a specific location, select the desired country: