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Native Aerobacter aerogenes Sulfatase

Cat No.
NATE-0686
Description
Sulfatases EC 3.1.6.1 are enzymes of the esterase class that catalyze the hydrolysis of sulfate esters. These may be found on a range of substrates, including steroids, carbohydrates and proteins. Sulfate esters may be formed from various alcohols and amines. In the latter case the resultant N-sulfates can also be termed sulfamates. Sulfatases play important roles in the cycling of sulfur in the environment, in the degradation of sulfated glycosaminoglycans and glycolipids in the lysosome, and in remodelling sulfated glycosaminoglycans in the extracellular space. Together with sulfotransferases, sulfatases form the major catalytic machinery for the synthesis and breakage of sulfate esters.
Abbr
Sulfatases, Native (Aerobacter aerogenes)
Alias
sulfatase
Source
Aerobacter aerogenes
Applications
Sulfatase from Aerobacter aerogenes has been used in a study to assess formation of tyrosine O-sulfate by mit ochondria and chloroplasts of Euglena.
Form
buffered aqueous glycerol solution; Solution in 50% glycerol containing 0.01 M Tris, pH 7.5.
Enzyme Commission Number
EC 3.1.6.1
Activity
2-5 units/mg protein (biuret), 10-20 units/mL
CAS No.
9016-17-5
Concentration
10-20 units/mL
Unit Definition
One unit will hydrolyze 1.0 μmole of p-nitrophenyl sulfate per min at pH 7.1 at 37°C.
Storage
−20°C
Synonyms
EC 3.1.6.1; 9016-17-5; sulfatase; nitrocatechol sulfatase; phenolsulfatase; phenylsulfatase; p-nitrophenyl sulfatase; arylsulfohydrolase; 4-methylumbelliferyl sulfatase; estrogen sulfatase; arylsulfatase
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sulfatase -Enzymatic Assay Protocol
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