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Native Almonds Mandelonitrile Lyase


Cat No.
NATE-0557
Description
In enzymology, a mandelonitrile lyase is an enzyme that catalyzes the chemical reaction:mandelonitrile↔ hydrogen cyanide + benzaldehyde. Hence, this enzyme has one substrate, mandelonitrile, and two products, hydrogen cyanide and benzaldehyde. This enzyme belongs to the family of lyases, specifically the aldehyde-lyases, which cleave carbon-carbon bonds. This enzyme participates in cyanoamino acid metabolism. It has 2 cofactors:flavin, and flavoprotein.
Abbr
PhaMDL, Native (Almonds)
Alias
PaHNL; AtHNL; PhaMDL
Source
Almonds
Applications
Mandelonitrile lyase from almonds has been used in a study to assess the apoplastic antioxidant system in Prunus. It has also been used in a study to investigate screening for new hydroxynitrilases from plants.
Product Overview
Mandelonitrile lyase is a cyanogenic enzyme.
Form
ammonium sulfate suspension; Suspension in 50 mM imidazole, 2.8 M (NH4)2SO4, pH 6.0
Enzyme Commission Number
EC 4.1.2.10
Activity
80-240 units/mg protein (biuret)
CAS No.
9024-43-5
Unit Definition
One unit will form 1.0 μmole of benzaldehyde and HCN from mandelonitrile per min at pH 5.4 at 25°C.
Storage
2-8°C
Synonyms
mandelonitrile lyase; EC 4.1.2.10; (R)-oxynitrilase; oxynitrilase; D-oxynitrilase; D-α-hydroxynitrile lyase; mandelonitrile benzaldehyde-lyase; PaHNL; AtHNL; PhaMDL; (R)-HNL; (R)-PeHNL; (R)-hydroxynitrile lyase; R-selective hydroxynitrile lyase; R-selective HNL; (R)-(+)-mandelonitrile lyase; 9024-43-5
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Product Name
EC No.
CAS No.
Source
Price
CatalogEXWM-4850
EC No.EC 4.1.2.10
CAS No.9024-43-5
Source
Related Protocols
mandelonitrile lyase -Enzymatic Assay Protocol
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