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Native Bacillus licheniformis Proteinase

Cat No.
NATE-0639
Description
Proteinase catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate.
Abbr
Proteinase, Native (Bacillus licheniformis)
Alias
protease
Source
Bacillus licheniformis
Applications
The enzyme from Creative Enzymes has been used to optimize release of all mit ochondrial populations from homogenized ventricular tissue of rat heart. It has also been used in the pre-hybridisation treatment of formalin fixed, paraffin wax-embedded liver specimens for detecting human and viral DNA. This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. This product also known as Subtilisin Carlsberg, has been used to hydrolyze cardiac cells to study the silencing of cardiac mit ochondrial NHE1.
Product Overview
Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and has a molecular weight of 27 KDa.
Form
lyophilized powder
Enzyme Commission Number
EC 3.4.21.62
Activity
7.0-14.0 units/mg solid
CAS No.
9001-92-7
Molecular Weight
27 KDa
Purity
crystallization
Specificity
Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.
Unit Definition
One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37°C (color by Folin-Ciocalteu reagent).
Storage
−20°C
Warnings
Amino acid analysis and isoelectric fecusing electrophoresis consistent with subtilisin Carlsberg.
Synonyms
protease; peptidase; proteinase; EC 3.4.21.62; 9014-01-1; Alkaline Protease; Protease from Bacillus licheniformis; Proteinase from Bacillus licheniformis; Subtilo peptidase A
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ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-0638
EC No.
CAS No.9001-92-7
SourceAspergillus melleus
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