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Native Bacillus sp Chitosanase

Cat No.
NATE-1746
Description
Chitosanase is a powdered chitosanase preparation made by submerged fermentation of a selected strain of the bacterium Bacillus sp. The enzyme catalyzes the breakdown of chitosan, a partially or completely de-acetylated derivative of chitin (β-1,4 homopolymer of N-acetyl glucosamine).
Abbr
Chitosanase, Native (Bacillus sp)
Alias
Chitosanase
Source
Bacillus sp
Applications
Chitosanase can be used for hydrolyzing chitosan(degree of de-acetylatin: 40~100%). Especially, it can be used for the production of chitosan oligosaccharides from chitosan, which have a variety of biological activities such as immuno-stimulating activity, anti-tumor activity, anti-microbial activity, etc.
Appearance
White or light yellow colored, freeze-dried powder
Enzyme Commission Number
EC 3.2.1.132
Activity
35,000U/g
CAS No.
51570-20-8
Molecular Weight
45,000Da estimated by SDS-PAGE
pH Stability
Stable in pH range of 4.5 to 8.0
Specificity
Shows high activities against chitosan substrates which are de-acetylated by 40 to 100%.
Unit Definition
The standard activity is determined by modified Schales method. One unit(U) is defined as the amount of enzyme that releases one ㎛ole of reducing sugar (measured as D-glucosamine equivalents) from chitosan per minute at pH 5.0 at 48°C. A detailed description of the method is available on request.
Optimum pH
pH range of 4.5 to 6.0
Optimum temperature
60°C
Thermal stability
More than 90% activity remains after 24 hr incubation at 40°C.
Storage
The product should be stored in a cool, dry environment with temperatures below 4°C.
Synonyms
Chitosanase; EC 3.2.1.132; 51570-20-8; Chitosan N-acetylglucosaminohydrolase
Notes
Arsenic: Less than 4ppm.
Heavy metals: Less than 10ppm (as Pb).
Coli-form bacteria: Less than 30 colony-forming units(CFU) per gram.
Samonella: Negative.
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