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Native Bacillus stearothermophilus Polynucleotide Phosphorylase

Cat No.
NATE-1908
Description
Polynucleotide phosphorylase (PNPase) is a bifunctional enzyme with a phosphorolytic 3' to 5' exoribonuclease activity and a 3'-terminal oligonucleotide polymerase activity. It is also involved in mRNA processing and degradation in bacteria, plants, and humans.
Abbr
PNPase, Native (Bacillus stearothermophilus)
Alias
PNPase
Source
Bacillus stearothermophilus
Applications
The enzyme is useful for the preparation of polyribonucleotide.
Appearance
Lyophilized
Enzyme Commission Number
EC 2.7.7.8
CAS No.
9014-12-4
Molecular Weight
300,000 - 340,000; Subunit molecular weight : ca. 85,000.
Isoelectric point
4
pH Stability
9.0 - 11.0
Michaelis Constant
(38 mM Tris-HCI buffer, pH 9.5, at 60 °C)
Poly A: 0.27 mM**;
KH2PO4: 3.0 mM;
**concentration of poly A was calculated as AMP concentration.
Unit Definition
One unit of activity is defined as the amount of PNPase that forms 1 μmol of ADP per hour at 60 °C by depolymerizing of Poly A.
Optimum pH
9.0 - 9.5
Thermal stability
No detectable decrease in activity up to 55 °C.
Storage
Stable at -20 °C for at least one year.
Synonyms
PNPase; nucleoside diphosphate:polynucleotidyl transferase; polyribonucleotide nucleotidyltransferase; polynucleotide phosphorylase; polyribonucleotide phosphorylase; EC 2.7.7.8; 9014-12-4
Specific Activity
more than 2,000 U/mg protein
Reaction
RNAn+1 + Pi ←→ RNAn + Nucleoside diphosphate
Notes
Effectors: cations and anions.
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Related Protocols
polynucleotide phosphorylase -Enzymatic Assay Protocol
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