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Native Bovine Phospholipase A2

Cat No.
NATE-0583
Description
Phospholipase A2 (PLA2) hydrolyzes the β-ester bond of zwitterionic glycerophospholipids. Preferred substrates are phosphatidylcholine, phosphatidylethanolamine, and their plasmalogen analogues. Phosphatidylinositol and phosphatidylserine are also hydrolyzed. It aggressively attacks phospholipids in membranes of intact cells. PLA2 specifically recognizes the sn-2 acyl bond of phospholipids and catalytically hydrolyzes the bond releasing arachidonic acid and lysophospholipids.
Abbr
PLA2, Native (Bovine)
Alias
PLA2; PLA2s
Source
Bovine pancreas
Species
Bovine
Form
lyophilized powder; Contains Tris buffer salts.
Enzyme Commission Number
EC 3.1.1.4
Activity
> 20 units/mg protein
CAS No.
9001-84-7
Unit Definition
One unit will hydrolyze 1.0 μmole of soybean L-α-phosphatidylcholine to L-α-lysophosphatidylcholine and a fatty acid per min at pH 8.0 and 37°C.
Storage
−20°C
Warnings
Protein determined by biuret.
Pathway
Acyl chain remodelling of PC, organism-specific biosystem; Arachidonic acid metabolism, organism-specific biosystem; Ether lipid metabolism, conserved biosystem
Function
calcium ion binding; phospholipase A2 activity
Synonyms
Phospholipases A2; EC 3.1.1.4; 9001-84-7; lecithinase A; phosphatidase; phosphatidolipase; phospholipase A; PLA2; Phosphatidylcholine 2-acylhydrolase; PLA2s
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogPLA2-001
EC No.
CAS No.
Source
CatalogNATE-0584
EC No.EC 3.1.1.4
CAS No.9001-84-7
SourceCrotalus durissus terrificus venom
CatalogNATE-0585
EC No.EC 3.1.1.4
CAS No.9001-84-7
SourceHoney bee venom (Apis mellifera)
CatalogNATE-0586
EC No.EC 3.1.1.4
CAS No.9001-84-7
SourceNaja mossambica mossambica
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Realted Protocols
PHOSPHOLIPASE A2 -Enzymatic Assay Protocol
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