Products

Enzymes for Research, Diagnostic and Industrial Use

Native Fig tree latex Ficin

Cat No.
NATE-0255
Description
Ficin is classified as a thiol protease. It contains a single reactive cysteine at its active site. The amino acid homology of the active site is similar to that of papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported Km for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. Ficin is inhibited by iodoacetamide, iodoacetic acid, N-ethylmaleimide, mercuric chloride, DFP (diisopropyl fluorophosphate), TLCK (Na-p-Tosyl-lysine chloromethyl ketone), and TPCK (N-Tosyl-L-phenylalanine chloromethyl ketone). Ficin can be used to generate high yielding F (ab')2 fragments from mouse IgG1.
Abbr
Ficin, Native (Fig tree latex)
Alias
ficin; debricin
Source
Fig tree latex
Form
saline suspension or lyophilized powder
Enzyme Commission Number
EC 3.4.22.3
Activity
> 1.0 units/mg protein
CAS No.
9001-33-6
Molecular Weight
23.8 kDa
Specificity
Extinction Coefficient: E1% = 21.0 (280 nm) pI: 9.0
Unit Definition
One unit will produce a ΔA280 of 1.0 per min at pH 7.0 at 37°C when measuring TCA soluble products from casein in a final volume of 10 mL (1 cm light path).
Synonyms
ficin; debricin; higueroxyl delabarre; EC 3.4.22.3; 9001-33-6; ficain
Download Datasheet:


“Ficin” Total Products Page

Inquiry
Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-1865
EC No.EC 3.4.22.3
CAS No.
Source
CatalogEXWM-4204
ProductNameficain
EC No.EC 3.4.22.3
CAS No.9001-33-6
Source
Realted Protocols
BROMELAIN -Enzymatic Assay Protocol
Online Inquiry
Name:
*Phone:
*E-mail Address:
Country:
Company/Institution:
*Products or Services Interested:
Quantity:
Project Description:
Our Products are Not Intended for Private Therapeutic Use!
*Verification Code:
Please input "enzymes"(case insensitive) as verification code.

Welcome! For price inquiries, please feel free to contact us through the form below through the form on the left side. We will get back to you as soon as possible.

Sitemap  Privacy Policy
Copyright ©2011 - 2017 Creative Enzymes.
Contact Us 45-1 Ramsey Road, Shirley, NY 11967, USA
Email: info@creative-enzymes.com
Tel: 1-631-562-8517 1-631-448-7888
Fax: 1-631-938-8127
Distributors To view the contact information for a specific location, select the desired country: