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Native Gluconobacter industrius D-Fructose Dehydrogenase


Cat No.
NATE-0184
Description
D-fructose dehydrogenase is a heterotrimeric membrane-bound enzyme commonly seen in various Gluconobacter sp. especially in Gluconobacter japonicus (Gluconobacter industrius). It has a molecular mass of ca. 140 kDa, consisting of subunits I (67kDa), II (51 kDa), and III (20 kDa) and catalyzes the oxidation of D-fructose to produce 5-keto-D-fructose. The enzyme is a flavoprotein-cytochrome c complex with subunits I and II covalently bound to flavin adenine dinucleotide (FAD) and heme C as prosthetic groups, respectively.
Abbr
D-Fructose Dehydrogenase, Native (Gluconobacter industrius)
Source
Gluconobacter industrius
Applications
D-fructose dehydrogenase is used as a biosensor to detect the presence of D-fructose. Fructose dehydrogenase (FDH) is used in a number of basic research projects to examine the electrochemical properties of enzyme-catalyzed electrode reactions called bioelectrocatalysis. D-fructose dehydrogenase has been used in a study that contributed towards a convenient method for measuring rare sugars, monosaccharides, for applications in the bio-industry. A direct electron transfer reaction of d-fructose dehydrogenase adsorbed on a porous carbon electrode surface has been used to describe a batch-type coulometric d-fructose biosensor.
Form
Lyophilized powder
Enzyme Commission Number
EC 1.1.99.11
Activity
400-1,200 units/mg
CAS No.
37250-85-4
Composition
Protein, <10% Lowry
Unit Definition
One unit will convert 1.0 μmole D-fructose to 5-ketofructose per min at pH 4.5 at 37°C.
Storage
−20°C
Synonyms
EC 1.1.99.11; fructose 5-dehydrogenase; D-fructose dehydrogenase; D-fructose:(acceptor) 5-oxidoreductase; 37250-85-4
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Product Name
EC No.
CAS No.
Source
Price
CatalogNATE-0185
EC No.EC 1.1.99.11
CAS No.37250-85-4
SourceGluconobacter sp.
CatalogEXWM-0444
EC No.EC 1.1.99.11
CAS No.37250-85-4
Source
Related Services
Related Protocols
d-fructose dehydrogenase -Enzymatic Assay Protocol
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