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Native Helix pomatia β-Glucuronidase

Cat No.
NATE-0331
Description
β-glucuronidase catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.
Abbr
GUSB, Native (Helix pomatia)
Alias
GUSB
Source
Helix pomatia
Applications
Clinical Testing Diagnostic Assay Manufacturing
Product Overview
Type H-1, partially purified powder, > 300 kDa units/g solid; Type H-5, lyophilized powder, > 400 kDa units/g solid; Type H-3, aqueous solution, > 90 kDa units/mL; Type H-3AF, aqueous solution, > 60 kDa units/mL; Type H-2, aqueous solution, > 85 kDa units/mL; Type HP-2, aqueous solution, > 100 kDa units/mL
Form
partially purified powder or Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.
Enzyme Commission Number
EC 3.2.1.31
CAS No.
9001-45-0
Unit Definition
One unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37°C at pH 5.0 (30 min assay). Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37°C.
Optimum pH
4.5 to 5.0
Storage
Store at −20°C.
Inhibitors
D-glucuronic acid, D-galacturonic acid, D-glucaro-1, 4-lactone
Synonyms
β-glucuronide glucuronohydrolase glucuronidase; exo-β-D-glucuronidase; ketodase; EC 3.2.1.31; 9001-45-0; β-D-glucuronoside glucuronosohydrolase; GUSB
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