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Native Helix pomatia Sulfatase

Cat No.
NATE-0687
Description
Sulfatases EC 3.1.6.1 are enzymes of the esterase class that catalyze the hydrolysis of sulfate esters. These may be found on a range of substrates, including steroids, carbohydrates and proteins. Sulfate esters may be formed from various alcohols and amines. In the latter case the resultant N-sulfates can also be termed sulfamates. Sulfatases play important roles in the cycling of sulfur in the environment, in the degradation of sulfated glycosaminoglycans and glycolipids in the lysosome, and in remodelling sulfated glycosaminoglycans in the extracellular space. Together with sulfotransferases, sulfatases form the major catalytic machinery for the synthesis and breakage of sulfate esters.
Abbr
Sulfatases, Native (Helix pomatia)
Alias
sulfatase
Source
Helix pomatia
Applications
Sulfatase from Helix pomatia has been used in a study to develop a bioassay based screening of steroid derivatives in animal feed and supplements. Sulfatase from Helix pomatia has also been used in a study to inform new aspects of 17α-estradiol metabolism in man.
Form
Type I, powder; Type II, aqueous solution.
Enzyme Commission Number
EC 3.1.6.1
Activity
Type I, > 10 ,000 units/g solid; Type II, > 2 ,000 units/mL.
CAS No.
9016-17-5
Unit Definition
One unit will hydrolyze 1.0 μmole of p-nitrocatechol sulfate per hour at pH 5.0 at 37°C (30 min assay).
Storage
−20°C
Synonyms
EC 3.1.6.1; 9016-17-5; sulfatase; nitrocatechol sulfatase; phenolsulfatase; phenylsulfatase; p-nitrophenyl sulfatase; arylsulfohydrolase; 4-methylumbelliferyl sulfatase; estrogen sulfatase; arylsulfatase
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Source
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sulfatase -Enzymatic Assay Protocol
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