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Enzymes for Research, Diagnostic and Industrial Use

Native Leuconostoc mesenteroides 6-Phosphogluconolactonase

Cat No.
NATE-0889
Description
6-Phosphogluconolactonase is an enzyme in the pentose phosphate pathway. It converts 6-phosphogluconolactone to 6-phosphogluconate.
Abbr
6-Phosphogluconolactonase, Native (Leuconostoc mesenteroides)
Source
Leuconostoc mesenteroides
Species
Leuconostoc mesenteroides
Applications
Use 6-Phosphogluconolactonase in diagnostic tests for the determination of creatine kinase or glucose in the combination with Hexokinase, Glucose-6-phosphate Dehydrogenase and Phosphogluconate Dehydrogenase.
Appearance
White lyophilizate
Product Overview
Hydrolase that catalyzes the conversion of 6-phosphogluconolactone to 6-phosphogluconate. Increase the sensitivity of your Creatine Kinase assay.
Activity
>50 U/mg
Contaminants
Creatine kinase: <0.001 G6P-DH: <0.02 Myokinase: <0.001 "NADPH oxidase": <0.001 6- Phosphogluconate dehydrogenase: <0.01
Molecular Weight
38 kD (SDS)
Isoelectric point
6
pH Stability
7.0-9.0
Michaelis Constant
(MES buffer, pH 6.5; +25°C): 6-Phosphogluconalactone: < 1 x 10-7 mol/l
Specificity
6-Phosphogluconolactone 100%, gluconolactone 0.5%
Optimum pH
6.0-7.5
Stability
At -15 to -25°C within specification range for 12 months.
Inhibitors
(NH4)2SO4 (> 20 mmol/l), Mg2+ (>10 mmol/l), NaCl (>10 mmol/l). The enzyme is not inhibited by Cu2+, Zn2+, EDTA, 5.5'-dithiobis-2-nitrobenzoic acid, octanol (0.01%), Triton X-100 (1%) and Thesit (1%).
Synonyms
6-Phosphogluconolactonase
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