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Enzymes for Research, Diagnostic and Industrial Use

Native Microorganism Cholesterol Oxidase

Cat No.
DIA-138
Description
Recombinant Cholesterol Oxidase belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. This enzyme participates in bile acid biosynthesis.
Abbr
Cholesterol Oxidase (Microorganism)
Source
Microorganism
Applications
This enzyme is useful for enzymatic determination of cholesterol in serum when coupled with cholesterol esterase in clinical analysis.
Appearance
Yellowish amorphous powder, lyophilized
Enzyme Commission Number
EC 1.1.3.6
Activity
GradeIII 12U/mg-solid or more
CAS No.
9028-76-6
Contaminants
Catalase < 1.0×10⁻¹% Cholesterol esterase < 1.0×10⁻²%
Molecular Weight
approx. 55 kDa (by gel-filtration)
pH Stability
pH 5.0-10.0 (25°C, 20hr)
Michaelis Constant
3.0×10⁻⁵M(Cholesterol)
Optimum pH
7.0-8.0
Optimum temperature
60°C
Thermal stability
below 60°C (pH 7.0, 15min)
Stability
Stable at-20°C for at least 9 months
Stabilizers
Bovine serum albumin, amino acids
Inhibitors
Ionic detergents, Hg⁺⁺
Synonyms
Cholesterol-O2 oxidoreductase; 3 beta-Hydroxy steroid oxidoreductase; 3β-hydroxysteroid: oxygen oxidoreductase; cholesterol: oxygen oxidoreductase; cholesterol oxidase; EC 1.1.3.6
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogDIA-136
EC No.EC 1.1.3.6
CAS No.9026-00-0
SourcePseudomonas sp.
CatalogDIA-284
EC No.
CAS No.
SourceBrevibacterium species
CatalogDIA-406
EC No.EC 1.1.3.6
CAS No.9028-76-6
SourceE. coli
CatalogDIA-408
EC No.EC 1.1.3.6
CAS No.9028-76-6
SourceE. coli
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Realted Protocols
CHOLESTEROL OXIDASE -Enzymatic Assay Protocol
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