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Native Microorganism Glucose Dehyrogenase (NAD(P)-dependent)


Cat No.
DIA-191
Description
In enzymology, a glucose 1-dehydrogenase (EC 1.1.1.47) is an enzyme that catalyzes the chemical reaction: beta-D-glucose + NAD(P)+ ↔ D-glucono-1,5-lactone + NAD(P)H + H+. The 3 substrates of this enzyme are beta-D-glucose, NAD+, and NADP+, whereas its 4 products are D-glucono-1,5-lactone, NADH, NADPH, and H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor.
Abbr
Glucose Dehyrogenase (Microorganism)
Source
Microorganism
Applications
This enzyme is useful for enzymatic determination of D-Glucose.
Appearance
White amorphous powder, lyophilized
Form
Freeze dried powder
Enzyme Commission Number
EC 1.1.1.47
Activity
GradeⅢ 250U/mg-solid or more
CAS No.
9028-53-9
Contaminants
NADH oxidase < 1.0×10⁻³% α-Glucosidase < 1.0×10⁻³% Glucose-6-phosphate dehydrogenase < 1.0×10⁻³%
Molecular Weight
approx. 101 kDa (Gel filtration)
Isoelectric point
4.5
pH Stability
pH 6.0-7.5 (20°C, 16hr)
Michaelis Constant
NAD⁺linked : 1.38×10⁻²M (D-Glucose) 3.09×10⁻⁴M (NAD⁺), NADP⁺linked : 1.25×10⁻²M (D-Glucose) 4.07×10⁻⁵M (NADP⁺)
Specificity
Specific for ß-D,-Glucose or 2-Deoxy-glucose (Either NAD⁺ or NADP⁺ serves as coenzyme.)
Optimum pH
9
Optimum temperature
55°C
Thermal stability
45°C (15min-treatment with 50mM K-phosphate buffer, pH 7.0)
Stability
Stable at-20°C for at least one year
Inhibitors
Ag⁺, Hg²⁺, Monoiodoacetate
Synonyms
Glucose Dehyrogenase; EC 1.1.1.47; beta-D-glucose: NAD(P)+ 1-oxidoreductase; D-glucose dehydrogenase (NAD(P)+)
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Related Services
Related Protocols
GLUCOSE DEHYDROGENASE -Enzymatic Assay Protocol
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