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Native Microorganism Mutarotase

Cat No.
NATE-1907
Description
In enzymology, an aldose 1-epimerase (EC 5.1.3.3) is an enzyme that catalyzes the chemical reaction:alpha-D-glucose↔ beta-D-glucose. Hence, this enzyme has one substrate, alpha-D-glucose, and one product, beta-D-glucose. This enzyme belongs to the family of isomerases, specifically those racemases and epimerases acting on carbohydrates and derivatives. This enzyme participates in glycolysis and gluconeogenesis.
Abbr
Mutarotase, Native (Microorganism)
Alias
Mutarotase
Source
Microorganism
Applications
This enzyme is useful for enzymatic determination of glucose.
Appearance
Lyophilized
Enzyme Commission Number
EC 5.1.3.3
CAS No.
9031-76-9
Contaminants
(as MRO activity = 100 %)
NADHoxidase: < 0.01 %
Molecular Weight
ca. 39,500
pH Stability
3.5 - 10.0
Unit Definition
One unit of activity is defined as the amount of Mutarotase that forms 10μmol of NADH per minute at 25 °C.
Optimum pH
7.0 - 9.0
Thermal stability
No detectable decrease in activity up to 50 °C.
Storage
Stable at -20 °C for at least one year.
Synonyms
mutarotase; aldose mutarotase; galactose mutarotase; galactose 1-epimerase; D-galactose 1-epimerase; aldose 1-epimerase; EC 5.1.3.3
Specific Activity
more than 120 U/mg protein
Reaction
α-D-glucose ←→ β-D-glucose
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