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Enzymes for Research, Diagnostic and Industrial Use

Native Pediococcus sp. Glycerol 3-phosphate Oxidase

Cat No.
NATE-0315
Description
In enzymology, a glycerol-3-phosphate oxidase (EC 1.1.3.21) is an enzyme that catalyzes the chemical reaction:sn-glycerol 3-phosphate + O2↔ glycerone phosphate + H2O2. Thus, the two substrates of this enzyme are sn-glycerol 3-phosphate and O2, whereas its two products are glycerone phosphate and H2O2. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. This enzyme participates in glycerophospholipid metabolism. It employs one cofactor, FAD.
Abbr
GPO, Native (Pediococcus sp.)
Source
Pediococcus sp.
Applications
This enzyme is useful for enzymatic determination of triglyceride when coupled with lipoprotein lipase and glycerokinase in clinical analysis.
Form
Lyophilized powder containing stabilizers
Enzyme Commission Number
EC 1.1.3.21
Activity
40-80 units/mg solid
CAS No.
9046-28-0
Molecular Weight
~76 kDa (gel filtration)
Isoelectric point
4.1-/+0.1
pH Stability
6.5-8.5 (25°C, 20hr)
Michaelis Constant
3.2x10-3M (L-α-Glycerophosphate), 6.8 x 10-3M (D, L-form)
Unit Definition
One unit will oxidize 1.0 μmole of L-glycerol 3-phosphate to dihydroxyacetone phosphate with the formation of H2O2 per min at 37°C, at the appropriate pH.
Optimum pH
35-40°C
Thermal stability
below 40°C (pH 7.0, 15min)
Storage
2-8°C
Inhibitors
Ionic detergents (SDS, LBS, etc.), Hg++, Ag+
Synonyms
EC 1.1.3.21; glycerol phosphate oxidase; glycerol-1-phosphate oxidase; glycerol phosphate oxidase; L-α-glycerophosphate oxidase; α-glycerophosphate oxidase; L-α-glycerol-3-phosphate oxidase; Glycerol 3-phosphate Oxidase; 9046-28-0; sn-Glycerol 3-phosphate:oxygen 2-oxidoreductase; L-Glycerol 3-phosphate Oxidase; GPO
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“Glycerol-3-phosphate oxidase” Total Products Page

Inquiry
Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogDIA-154
EC No.EC 1.1.3.21
CAS No.9046-28-0
SourcePediococcus sp.
CatalogDIA-199
EC No.EC 1.1.3.21
CAS No.9046-28-0
SourcePediococcus sp.
CatalogDIA-200
EC No.EC 1.1.3.21
CAS No.9046-28-0
SourceMicroorganism
CatalogDIA-286
EC No.
CAS No.9046-28-0
SourceE. coli
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Realted Protocols
GLYCEROL-3-PHOSPHATE OXIDASE -Enzymatic Assay Protocol
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