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Native Rabbit Aldolase

Cat No.
NATE-0048
Description
Fructose-bisphosphate aldolase (EC 4.1.2.13), often just aldolase, is an enzyme catalyzing a reversible reaction that splits the aldol, fructose 1,6-bisphosphate, into the triose phosphates dihydroxyacetone phosphate (DHAP) and glyceraldehyde 3-phosphate (G3P). Aldolase can also produce DHAP from other (3S,4R)-ketose 1-phosphates such as fructose 1-phosphate and sedoheptulose 1,7-bisphosphate. Gluconeogenesis and the Calvin cycle, which are anabolic pathways, use the reverse reaction. Glycolysis, a catabolic pathway, uses the forward reaction. Aldolase is divided into two classes by mechanism.
Abbr
Aldolase, Native (Rabbit)
Alias
Aldolase
Source
Rabbit muscle
Species
Rabbit
Applications
Aldolase is used to convert fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. Aldolase, from rabbit muscle has been used for stereospecific deprotonation at DHAP C3. This product is essentially sulfate-free and contains Citrate buffer salts.
Form
lyophilized powder. Essentially sulfate-free containing Citrate buffer salts
Enzyme Commission Number
EC 4.1.2.13
Activity
Type I, lyophilized powder, > 8.0 units/mg protein; Type II, ammonium sulfate suspension, 10-20 units/mg protein
CAS No.
9024-52-6
Unit Definition
One unit will convert 1.0 μmole of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate per min at pH 7.4 at 25°C.
Storage
−20°C
Synonyms
aldolase; fructose-1,6-bisphosphate triosephosphate-lyase; Fructose-bisphosphate aldolase; fructose diphosphate aldolase; D-fructose-1,6-bisphosphate D-glyceraldehyde-3-phosphate-lyase; EC 4.1.2.13; 9024-52-6
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Realted Protocols
aldolase -Enzymatic Assay Protocol
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