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Native Saccharomyces cerevisiae Glyoxalase I

Cat No.
NATE-0308
Description
Glyoxalase I is universally expressed and involved in the protection against cellular damage due to cytotoxic metabolites such as advanced glycation end products (AGEs). It is an integral component of the detoxification system, catalyzing the conversion of reactive, acyclic a-oxoaldehydes into the corresponding a-hydroxyacids in a glutathione-dependent manner.
Abbr
Glyoxalase I, Native (Saccharomyces cerevisiae)
Source
Saccharomyces cerevisiae
Product Overview
Glyoxalase detoxification system consists of glyoxalase (GLO)-I and GLO-II. GLO-I is a cytosolic, 42 kDa, dimeric Zn2+ metalloenzyme.
Form
buffered aqueous glycerol solution; Solution in 50% glycerol, 0.4 M (NH4)2SO4 and 0.002 M KH2PO4 pH 6.5
Enzyme Commission Number
EC 4.4.1.5
Activity
> 400 units/mg protein
CAS No.
9033-12-9
Molecular Weight
42 kDa
Unit Definition
One unit will form 1.0 μmole of S-lactoylglutathione from methylglyoxal and reduced glutathione per min at pH 6.6 at 25°C.
Storage
2-8°C
Warnings
Protein determined by biuret.
Synonyms
lactoylglutathione lyase; EC 4.4.1.5; methylglyoxalase; aldoketomutase; ketone-aldehyde mutase; glyoxylase I; (R)-S-lactoylglutathione methylglyoxal-lyase (isomerizing); 9033-12-9
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Related Products
Catalog
ProductName
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CatalogNATE-1651
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CAS No.
SourceE. coli
CatalogEXWM-5339
EC No.EC 4.4.1.5
CAS No.9033-12-9
Source
Related Protocols
glyoxalase_i -Enzymatic Assay Protocol
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