Description
Sterol esterase belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. The systematic name of this enzyme class is steryl-ester acylhydrolase. This enzyme participates in bile acid biosynthesis.
Abbr
Cholesterol Esterase (Schizophyllum commune)
Source
Schizophyllum commune
Applications
This enzyme is useful for enzymatic determination of total cholesterol when coupled with cholesterol oxidase in clinical analysis.
Appearance
Light brown amorphous powder, lyophilized
Enzyme Commission Number
EC 3.1.1.13
Activity
GradeIII 2.0 U/mg-solid or more (containing approx. 20% of stabilizers)
Molecular Weight
approx. 130 kDa
Isoelectric point
4.1±0.1
pH Stability
pH 2.5-7.5 (25°C, 20hr)
Michaelis Constant
3.9×10⁻⁵M (Linoleate), 9.2×10⁻⁵M (Palmitate), 6.3×10⁻⁵M (Decylate), 8.8×10⁻⁵M (Propionate)
Optimum pH
4.8-8.0 (Cholesterol linoleate), 5.0 (serum)
Optimum temperature
55-60°C
Thermal stability
below 55°C (pH 5.5, 10min)
Inhibitors
Heavy metal ions (Hg⁺⁺, Ag⁺, Fe⁺⁺⁺)
Synonyms
cholesterol esterase; cholesteryl ester synthase; triterpenol esterase; cholesteryl esterase; cholesteryl ester hydrolase; sterol ester hydrolase; cholesterol ester hydrolase; cholesterase; acylcholesterol lipase; EC 3.1.1.13; Sterol esterase
