Native Schizophyllum commune Cholesterol Esterase

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Cat No.

DIA-133

Description

Sterol esterase belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. The systematic name of this enzyme class is steryl-ester acylhydrolase. This enzyme participates in bile acid biosynthesis.

Abbr

Cholesterol Esterase (Schizophyllum commune)

Source

Schizophyllum commune

Applications

This enzyme is useful for enzymatic determination of total cholesterol when coupled with cholesterol oxidase in clinical analysis.

Appearance

Light brown amorphous powder, lyophilized

Enzyme Commission Number

EC 3.1.1.13

Activity

GradeIII 2.0 U/mg-solid or more (containing approx. 20% of stabilizers)

CAS_No

9026-00-0

Molecular Weight

approx. 130 kDa

Isoelectric point

4.1±0.1

pH Stability

pH 2.5-7.5 (25°C, 20hr)

Michaelis Constant

3.9×10⁻⁵M (Linoleate), 9.2×10⁻⁵M (Palmitate), 6.3×10⁻⁵M (Decylate), 8.8×10⁻⁵M (Propionate)

Optimum pH

4.8-8.0 (Cholesterol linoleate), 5.0 (serum)

Optimum temperature

55-60°C

Thermal stability

below 55°C (pH 5.5, 10min)

Stability

Store at -20°C

Stabilizers

Na-Cholate

Inhibitors

Heavy metal ions (Hg⁺⁺, Ag⁺, Fe⁺⁺⁺)

Synonyms

cholesterol esterase; cholesteryl ester synthase; triterpenol esterase; cholesteryl esterase; cholesteryl ester hydrolase; sterol ester hydrolase; cholesterol ester hydrolase; cholesterase; acylcholesterol lipase; EC 3.1.1.13; Sterol esterase

"Cholesterol Esterase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1679	Native Candida Rugosa Cholesterol Esterase	EC 3.1.1.13	9026-00-0	Candida Rugosa	Inquiry
DIA-134	Native Pseudomonas sp. Cholesterol Esterase	EC 3.1.1.13	9026-00-0	Pseudomonas sp.	Inquiry
DIA-135	Native Microorganism Cholesterol Esterase	EC 3.1.1.13	9026-00-0	Microorganism	Inquiry
DIA-281	Chemically modified Pseudomonas species Cholesterol Esterase			Pseudomonas species	Inquiry

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