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O-Glycosidase from Streptococcus pneumoniae, Recombinant

Cat No.
NATE-0497
Description
O-Glycosidase releases unsubstituted Ser-and Thr-linked β-Gal-(1→3)-α-GalNAc (Core 1 type O-glycan) from glycoproteins. Substitutions of the disaccharide core with sialic acid, lactosamine (galactose-N-acetyl glucosamine), or fucose will block hydrolysis and prevent the liberation of the oligosaccharide from the protein. Pretreament with glycolytic enzymes to remove substituent saccharides from the O-glycan may be needed prior to cleavage using O-glycosidase.
Abbr
O-Glycosidase, Recombinant (Streptococcus pneumoniae)
Source
E. coli
Species
Streptococcus pneumoniae
Package
Supplied with 5× Reaction Buffer, 250 mM NaH2PO4 pH 5.0.
Form
buffered aqueous solution; Solution in 50 mM sodium phosphate, pH 7.5
Enzyme Commission Number
EC 3.2.1.97
CAS No.
9032-92-2
Unit Definition
One unit will hydrolyze 1 μmole of p-nitrophenyl galacto-N-bioside (β-Gal-(1→3)-α-GalNAc-1→ΟC6H4NO2) per min at 37°C at pH 6.5.
Storage
2-8°C
Warnings
Screened for presence of: β-galactosidase, α-mannosidase, β-hexosaminidase, α-fucosidase, neuraminidase, and proteases. See Certificate of Analysis for lot specific information.
Synonyms
endo-α-acetylgalactosaminidase; endo-α-N-acetyl-D-galactosaminidase; mucinaminylserine mucinaminidase; D-galactosyl-3-(N-acetyl-α-D-galactosaminyl)-L-serine mucinaminohydrolase; endo-α-GalNAc-ase; glycopeptide α-N-acetylgalactosaminidase; D-galactosyl-N-acetyl-α-D-galactosamine D-galactosyl-N-acetyl-galactosaminohydrolase; 9032-92-2; EC 3.2.1.97
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-0905
EC No.EC 3.2.1.97
CAS No.59793-96-3
SourceEnterococcus faecalis
CatalogEXWM-3960
EC No.EC 3.2.1.97
CAS No.59793-96-3
Source
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