Description
Pectinesterase catalyzes the hydrolysis of the methyl esters of pectin to yield pectate and methanol.
Abbr
Pectin methylesterase, Recombinant (Streptomyces avermitilis)
Source
Streptomyces avermitilis MA-4680
Form
Supplied in 3.2 M ammonium sulphate
Enzyme Commission Number
EC 3.1.1.11
Molecular Weight
39613.8 Da
Purity
> 90 % as judged by SDS-PAGE
Unit Definition
One unit is defined as the amount of enzyme required to release 1 microequivalent of acid from pectin (5 mg/mL 89 % methyl-esterified) per minute at pH 7.5 and 30oC, in an un-buffered assay containing 100 mM NaCl, as measured titrimetrically using 0.02 M NaOH.
Storage
Store at 4°C (shipped at room temperature)
Preparation Instructions
Agitate vial sufficiently to fully homogenise enzyme precipitate before use.
Synonyms
Pectinesterase; EC 3.1.1.11; pectin demethoxylase; pectin methoxylase; pectin methylesterase; pectase; pectin methyl esterase; pectinoesterase; pectin pectylhydrolase; 9025-98-3
