Pectinesterase catalyzes the hydrolysis of the methyl esters of pectin to yield pectate and methanol.
Pectin methylesterase, Recombinant (Streptomyces avermitilis)
Streptomyces avermitilis MA-4680
Supplied in 3.2 M ammonium sulphate
Enzyme Commission Number
> 90 % as judged by SDS-PAGE
One unit is defined as the amount of enzyme required to release 1 microequivalent of acid from pectin (5 mg/mL 89 % methyl-esterified) per minute at pH 7.5 and 30oC, in an un-buffered assay containing 100 mM NaCl, as measured titrimetrically using 0.02 M NaOH.
Store at 4°C (shipped at room temperature)
Agitate vial sufficiently to fully homogenise enzyme precipitate before use.
Pectinesterase; EC 188.8.131.52; pectin demethoxylase; pectin methoxylase; pectin methylesterase; pectase; pectin methyl esterase; pectinoesterase; pectin pectylhydrolase; 9025-98-3