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Polygalacturonase 4A from Bacillus subtilis, Recombinant

Cat No.
NATE-1507
Description
In enzymology, a galacturan 1,4-alpha-galacturonidase (EC 3.2.1.67) is an enzyme that catalyzes the chemical reaction: (1,4-alpha-D-galacturonide)n + H2O → (1,4-alpha-D-galacturonide)n-1 + D-galacturonate. Thus, the two substrates of this enzyme are (1,4-alpha-D-galacturonide)n and H2O, whereas its two products are (1,4-alpha-D-galacturonide)n-1 and D-galacturonate. This enzyme belongs to the family of hydrolases, specifically those glycosidases that hydrolyse O- and S-glycosyl compounds.
Abbr
Polygalacturonase 4A, Recombinant (Bacillus subtilis)
Source
E. coli
Species
Bacillus subtilis
Form
35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol
Enzyme Commission Number
EC 3.2.1.67
CAS No.
9045-35-6
Molecular Weight
51.5 kDa
Purity
>90% as judged by SDS-PAGE
Concentration
0.25 mg/mL
Specificity
Polygalacturonans and pNPαGalUA
Optimum pH
7.2-7.8
Optimum temperature
36.5 °C
Storage
This enzyme is shipped at room temperature but should be stored at -20 °C.
Synonyms
poly(1,4-alpha-D-galacturonide) galacturonohydrolase; exopolygalacturonase; poly(galacturonate) hydrolase; exo-D-galacturonase; exo-D-galacturonanase; exopoly-D-galacturonase; galacturan 1,4-alpha-galacturonidase; EC 3.2.1.67; Polygalacturonase
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CatalogEXWM-3931
EC No.EC 3.2.1.67
CAS No.9045-35-6
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