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Polynucleotide Phosphorylase from Escherichia coli, Recombinant

Cat No.
NATE-0608
Description
Polynucleotide phosphorylase (PNPase) is a bifunctional enzyme with a phosphorolytic 3' to 5' exoribonuclease activity and a 3'-terminal oligonucleotide polymerase activity. It is also involved in mRNA processing and degradation in bacteria, plants, and humans.
Abbr
PNPase, Recombinant (E. coli)
Alias
PNPase
Source
E. coli
Species
Escherichia coli
Applications
Polynucleotide phosphorylase (PNP) has been used in a study to show that spontaneous mutations resulting from replication errors are reduced in a PNP-deficient strain. It has also been used in a study to show that the absence of PNPase makes E. coli cells sensitive to UV, which suggests PNP has a role in survival of UV damage.
Form
Supplied as a solution in 20 mM Hepes buffer pH 7.9, 0.1 mM EDTA, 2 mM DTT, 12.5 mM MgCl2, 200 mM KCl, 21.4% (w/v) Glycerol
Enzyme Commission Number
EC 2.7.7.8
CAS No.
9014-12-4
Unit Definition
One unit will polymerize 1.0 μmole of ADP releasing 1.0 μmole of inorganic phosphate in 15 minutes, at pH 9.1 at 37°C.
Storage
−70°C
Synonyms
PNPase; nucleoside diphosphate:polynucleotidyl transferase; polyribonucleotide nucleotidyltransferase; polynucleotide phosphorylase; polyribonucleotide phosphorylase; EC 2.7.7.8; 9014-12-4
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-1908
EC No.EC 2.7.7.8
CAS No.9014-12-4
SourceBacillus stearothermophilus
CatalogNATE-0609
EC No.EC 2.7.7.8
CAS No.9014-12-4
SourceE. coli
CatalogNATE-0610
EC No.EC 2.7.7.8
CAS No.9014-12-4
SourceE. coli
CatalogEXWM-3292
EC No.EC 2.7.7.8
CAS No.9014-12-4
Source
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Realted Protocols
polynucleotide phosphorylase -Enzymatic Assay Protocol
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