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Enzymes for Research, Diagnostic and Industrial Use

Pup amidohydrolase

Cat No.
EXWM-4399
Description
The enzyme has been characterized from the bacterium Mycobacterium tuberculosis. It catalyses the hydrolysis of the amido group of the C-terminal glutamine of prokaryotic ubiquitin-like protein (Pup), thus activating it for ligation to target proteins, a process catalysed by EC 6.3.1.19, prokaryotic ubiquitin-like protein ligase. The reaction requires ATP as cofactor but not its hydrolysis. The enzyme also catalyses the hydrolytic cleavage of the bond formed by the ligase, between an ε-amino group of a lysine residue of the target protein and the γ-carboxylate of the C-terminal glutamate of the prokaryotic ubiquitin-like protein.
Form
Liquid or lyophilized powder
Enzyme Commission Number
Storage
Store it at +4 ºC for short term. For long term storage, store it at -20 ºC~-80 ºC.
Synonyms
dop (gene name); Pup deamidase; depupylase/deamidase; DPUP; depupylase
Reaction
[prokaryotic ubiquitin-like protein]-L-glutamine + H2O = [prokaryotic ubiquitin-like protein]-L-glutamate + NH3
Notes
This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
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